摘要
目的 :研究黄体酮 (P)对戊四唑 (PTZ)致痫大鼠的发作情况和对海马结构内的微管相关蛋白 2 (MAP2 )的影响。方法 :采用成年去卵巢 (OVX)雌性SD大鼠 ,设立空白对照组 ,实验对照组 ,实验给药组 ,取含背侧海马的脑片 ,进行免疫组化染色。结果 :(1)实验给药组大鼠均未出现癫痫发作 ,实验对照组大鼠发作均达Ⅳ级以上。 (2 )实验对照组海马结构内MAP2免疫阳性反应减弱 ,而实验给药组则增强。结论 :P可对抗PTZ致痫的行为发作 ,并且上调了海马内MAP2的免疫活性 ,可能参与了P对抗癫痫的机制。
Objective: To explore the effects of p rogesterone on pentylenetetrazol (PTZ) induced seizures and microtubule associated protein 2 (MAP2) of the hippocampus in ovariectomized female rats. Methods:Ovariectomized (OVX) adult females SD rats were randomly divided into NS control, experimental control and experimental groups. The brains were removed and slices were cut coronally using a vibrator. The slices with dorsal hippocampus were stained by ABC immunohistochemistry method. Results:(1)Pretreatment with progesterone protected OVX rats from PTZ-induced seizures completely, while rats of experimental control group all exhibited stage 4 or 5 seizures; (2) MAP-IR decreased in Hilus, molecular layer of dentate gyrus and CA1 of experimental control group compared with NS+NS group. However, compared with those of NS control group. MAP-IR increased in experimental group. Conclusion:Progesterone can protect OVX rats against PTZ-induced seizures, and it may enhance MAP2 immunohistochemistry in female rats, which may be one of mechanisms of P's anticonvulsant effects and protective action.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2005年第1期53-55,i004,共4页
Chinese Journal of Anatomy
基金
辽宁省科技厅博士启动基金 (2 0 0 1 1 0 2 0 82 )
