摘要
目的 探讨缝隙连接(GJ)蛋白Cx4 3在猫正常睫状体的表达分布以及外伤性前部增殖性玻璃体视网膜病变 (aPVR)模型中不同时间点、不同眼压 (IOP)下其表达位置和量的相应变化。方法 制作外伤性aPVR导致慢性低眼压的动物模型 ,监测受伤前后IOP ,并设立正常对照组 ,通过荧光免疫组织化学方法检测正常猫睫状体中Cx4 3蛋白的表达 ,进而分析 2 4h、3天、1周、2周和 4周时Cx4 3表达位置及量的变化。结果 Cx4 3分布在正常猫睫状体双层上皮之间 ,其构成的GJ连接色素上皮 (PE)与非色素上皮 (NPE) ;aPVR模型伤后 2 4hCx4 3表达明显增高 ,PE层内细胞间亦出现Cx4 3表达 ,1周后该蛋白于PE、NPE及层间广泛分布 ,且蛋白表达量达到最大值 ,2周、4周时蛋白表达量逐渐减少并局限分布在PE NPE层间。伤后 2 4hIOP略升高 ,此后逐渐下降 ;在伤后 3天内及 1周后Cx4 3表达量的变化趋势与IOP走向基本一致。结论 Cx4 3构成了猫睫状体PE与NPE之间的GJ通道 ;外伤性aPVR形成的低眼压与睫状体Cx4 3表达位置和量的变化关系密切。
Objective To investigate the expression of gap junction(GJ) protein Cx43 in normal cat ciliary body and its corresponding changes in location and quantity in traumatic anterior proliferative vitreoretinopathy (aPVR) model at different time points and under different intraocular pressure (IOP). Methods In a cat model of chronic hypotony after traumatic aPVR IOP was measured before and after trauma. Similar measurement was done in healthy cats. The location and content of Cx43 of ciliary epithelium was determined with immunohistochemistry technique separately in normal group and 24h, 3d, 1w, 2w and 4w after trauma in the experimental group. Results Cx43 was located between the bi-epithelium of ciliary body of the normal cat, forming GJ to communicate between PE and NPE. However, in the aPVR model, Cx43 appeared between PE cells also, and was up-regulated obviously at 24h, At 1w, Cx43 was seen to be distributed diffusely between all epithelial cells reaching the maximum level. Its level descended gradually after 2w and 4w. The IOP raised slightly after 24h and then gradually fall. The changes in Cx43 quantity was basically parallel to ΔIOP within 3d and after 1w. Conclusion In cat ciliary body Cx43 from the direct transportation channels for aqueous materials between PE and NPE. Its changes in location and quantity bore close relation ship with the formation of hypotony after aPVR.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2005年第2期107-110,共4页
Medical Journal of Chinese People's Liberation Army
基金
全军"十五"医学科研面上项目资助课题 (编号 0 1MA1 1 3)
