摘要
探讨组胺对人结肠肥大细胞类胰蛋白酶释放的调节作用。经酶消化后获取人结肠组织肥大细胞 ,激发后行多种干预实验。用酶联免疫吸附试验法测定类胰蛋白酶。结果发现组胺可诱导人结肠肥大细胞释放类胰蛋白酶 ,浓度为 10 0 μg/L时组胺释放量最大 ,为基础值的 3.5倍。浓度为 10 μg/L时对人肥大细胞的刺激强度与 10mg/L的抗IgE抗体相似。组胺的作用从加样后 10s开始 ,5min后完成。百日咳毒素和抗霉素A联合 2 脱氧 D 葡萄糖可显著抑制组胺诱导人结肠肥大细胞释放类胰蛋白酶。 10 0及 10 0 0 μg/L的组胺与抗IgE抗体或离子载体钙同时加入细胞中 ,诱导类胰蛋白酶释放的能力低于组胺单独作用组。结论为组胺可激活人结肠肥大细胞 。
To investigate the ability of histamine to modulate tryptase release by human colon mast cells. Human mast cells were dispersed from colon tissue with collagenase and hyaluronidase, and then challenged for 15 min at 37 ℃. Tryptase concentrations were measured with a sandwich ELISA procedure. We found that histamine was able to induce tryptase release from colon mast cells. The maximum release of tryptase was approximately 3.5 fold more than spontaneous release provoked by 100 μg/L histamine. As little as 10 μg/L of histamine showed a similar potency to that of anti-IgE( 10 mg/L) in the induction of tryptase release from colon mast cells. Histamine induced release of tryptase initiated at 10 sec when histamine was added to cells, gradually increased thereafter, and stoped at 5 min. Tryptase release induced by histamine was reduced to baseline level by pretreatment of colon mast cells with pertussis toxin or metabolic inhibitors. When 100 μg/L or 1 000 μg/L of histamine and anti-IgE or CI were added to colon mast cells at the same time, tryptase released was less than that induced by addition of the corresponding concentration of histamine alone. Our study shows that histamine is a potent activator of human colon mast cells, which represents a novel and pivotal self-amplification mechanism of mast cell degranulation.
出处
《基础医学与临床》
CSCD
北大核心
2005年第1期35-39,共5页
Basic and Clinical Medicine
基金
国家自然科学基金 (30 14 0 0 2 3)
广东省自然科学基金 (0 4 0 2 0 2 4 3)
李嘉诚基金 (C0 2 0 0 0 0 1)
