摘要
目的:探索卵圆细胞在参与实验性肝损伤修复过程中的作用和地位. 方法:清洁型SD大鼠随机分为正常组20只和实验组40只,各组于肝癌造模后的不同时段取肝组织标本进行常规病理和C-kit,PCNA免疫组化检测. 结果:正常组大鼠肝脏表面光滑,组织学形态正常, 偶见D-kit和PCNA阳性细胞.实验组于肝脏染毒2 wk, 首先于汇管区发现卵圆细胞沿胆管上皮依次排列增生, 这些卵圆细胞呈C-kit和PCNA阳性表达.随着染毒加重,大量卵圆细胞以汇管区为中心向肝小叶穿插生长, 正常肝细胞逐渐固缩、消亡.肝癌形成时,癌结节内外均见有卵圆细胞聚集. 结论:卵圆细胞在肝损伤修复中发挥主导作用;与肝癌的发生密切相关.
AIM: To explore the role of oval cells in progressing hepatic injury and repair as well as hepatocarcinogenesis. METHODS: SD rats were randomly divided into normal group(n = 20)and test group(n = 40). Hepatocarcinoma model was established by feeding the rats with 3, 3-diaminobenzidine(DAB). Pathological changes in the liver were examined under light microscope. The expression of c-kit and proliferating cell nuclear antigen(PCNA)were determined by immunohistochemistry. RESULTS: In the normal group, smooth surface and normal histology of the liver were observed, and little expression of c-kit and PCNA was detected. In the test group, oval cells with positive expression of c-kit and PCNA were first observed in the portal area two weeks after liver injury. The oval cells proliferated along with the bile duct epithelia. With the progress of hepatic injury, the oval cells extended into the centrilobular regions, and the liver cells gradually deflated and disappeared. When hepatocarcinoma occurred, a great number of oval cells were found inside and outside the cancer nodule. CONCLUSION: The oval cells play a leading role in the progressing hepatic injury and repair, and in the process of hepatocarcinogenesis.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第3期336-340,共5页
World Chinese Journal of Digestology
基金
广东省自然科学基金资助项目
No.2001.010593广东省重点自然科学基金资助项目
No.2002.020097~~
