摘要
[目的 ]研究N 甲基 N’ 硝基 N 甲基亚硝基胍 (MNNG)诱致小鼠胚胎畸形肢体基因表达的变化 ,筛选肢体畸形相关基因。 [方法 ]应用含有 8192条小鼠基因的cDNA表达谱芯片 ,对MNNG诱致的孕 16d胎鼠畸形肢体组织及正常肢体组织的基因表达谱进行分析。 [结果 ]畸形肢体组织共筛到差异表达基因 88条 ,其中下调 5 9条 ,上调 2 9条。 [结论 ]MNNG诱致的小鼠胚胎短肢、少趾畸形可有很多基因的表达改变 ,涉及与凋亡有关的基因、与生长因子或生长因子样物质有关的基因、结构基因及很多功能未知的相关基因 ,差异表达基因中以下调基因居多 ,这些结果可为进一步研究肢体短肢。
Objective To analyse the gene expression profiles in abnormal and normal developmental limbs of GD16 embryo from mouse dams exposed to MNNG. A series of expression microarray analysis of abnormal limb was initiated by cDNA microarray representing a set of 8192 mouse genes. Applying this cDNA microarray identified were 88 differentially expressed genes, among which 29 upregulated and 59 downregulated. [Conclusion] cDNA microarray for analysis of gene expression profile is a powerful method to identify teratogenicity-related genes. Further analysis of these differentially expressed genes will be helpful for understanding the molecular mechanism of teratogenicity.
出处
《环境与职业医学》
CAS
北大核心
2005年第1期1-3,共3页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金 (编号 :39870 657
30 0 70 662 )
