摘要
[目的 ]探讨硝酸稀土 [RE(NO3 ) 3 ]可否经乳汁分泌和 (或 )经胎盘转运进入仔代体内及对仔代免疫系统功能的影响。 [方法 ]妊娠小鼠在不同时期经口灌胃不同剂量 ( 2、2 0、2 0 0mg/kg)的RE(NO3 ) 3 :①妊娠 7～ 8d开始直至仔鼠离乳 ,即母鼠在妊娠期和哺乳期均接触RE(NO3 ) 3 ,共 3 5d(处理Ⅰ ) ;②妊娠母鼠生下仔鼠当天开始至仔鼠离乳 (哺乳期 )接触RE(NO3 ) 3 ,共 2 1d(处理Ⅱ ) ;③妊娠 7～ 8d开始至生下仔鼠 ,即母鼠只在妊娠期接触RE(NO3 ) 3 ,共 14d(处理Ⅲ )。对照组给予蒸馏水。分别检测刚出生仔鼠 (F1)及F1代仔鼠离乳时脾脏内稀土元素镧 (La)、铈 (Ce)、镨 (Pr)及钕 (Nd)的含量 ,同时观察母鼠不同时间染毒时F1代仔鼠离乳时细胞免疫、体液免疫及巨噬细胞功能的变化。 [结果 ]①处理Ⅲ 2 0 0mg/kg组La、Ce、Pr及Nd含量均明显高于对照组 ,差异具有显著性 (P <0 .0 5 )。②处理Ⅱ 2 0 0mg/kg剂量组F1代仔鼠刚离乳时脾脏内La、Ce、Pr及Nd含量均明显高于对照组。③F1代仔鼠刚离乳时脾抗体形成细胞数目 (IgM PFC/脾 )测定结果 :3种处理 2mg/kg剂量组分别较对照组升高 86.2 %、73 .8%和 82 .2 %。④F1代仔鼠刚离乳时迟发型变态反应 (DTH )测定结果 :处理Ⅰ 2 0 0mg/kg剂量组抑制率为 2 1.8% ,处理Ⅱ各剂?
Objective The focus of this study is to observe the effects of mixed rare earth nitrate(RE(NO 3) 3 on immune function of mice offspring and to explore whether these effects result from RE through the route of milk and placenta. RE(NO 3) 3 were continuously administered by gavage at the doses of 2,20,200 mg/kg during gestational and breast-feed period for 35 days(treatmentⅠ),only during breast-feed period for 21 days(treatmentⅡ) and only during gestational period for 14 days respectively(treatmentⅢ). The contents of La,Ce,Pr and Nd in fetuses and spleen of offspring,the weights of spleen and thymus,the spleen plaque forming cell(PFC),the charcoal clearance and the delayed type hypersensitivity(DTH) of offspring after weaning were determined respectively. 1)The contents of La,Ce,Pr and Nd in 200 mg/kg dose group were significantly higher than those of control group when RE(NO 3) 3 was given pregnant mice by oral administration during gestational period. 2)The contents of La,Ce,Pr and Nd in spleen in 200 mg/kg dose group were significantly higher than those of control group when given during breast-feed period.3) 2 mg/kg RE(NO 3) 3 could increases the number of IgM-PFC significantly by 86.2%,73.8% and 82.2% respectively in three treated groups. 4) In the treatment Ⅰexperiment 200 mg/kg RE(NO 3) 3 can induce 21.8% inhibition in DTH assay. It didn't show any difference in DTH in all treated groups compared to control.In treatment Ⅱexperiment,2 mg/kg RE(NO 3) 3 could significantly enhance the DTH(P<0.05) in treatment Ⅲ. 5)No significant differences were detected in the clearance index K and phagocytic index α between different dose groups in treatment Ⅰ experiment. In treatment Ⅱ experiment K and α were significantly higher than those of control in 20 mg/kg and 200 mg/kg dose group(P<0.05). At the dose of 2,20 mg/kg,they were significantly higher than those of control in treatment Ⅲ experiment(P<0.05). [Conclusion] From all the results,it was suggested that RE(NO 3) 3 can affect the immune function of mice offspring and these effect may via milk and placenta.
出处
《环境与职业医学》
CAS
北大核心
2005年第1期39-41,共3页
Journal of Environmental and Occupational Medicine
