摘要
将勐旺谷、云南药用野生稻和燕麦等外源供体材料的DNA,通过花粉管通道法和孕穗期茎注射法导入水稻栽培品种西南175,获得了一批性状变异并稳定遗传的材料。变异类型包括:生育期、株型、穗型、稻瘟病抗性、谷粒蛋白质和氨基酸含量、酯酶和过氧化物酶同工酶等。这些变异,有的为供体特有性状,有的则是新性状。从482份导入后代中,获得各类变异材料17份,变异率3.5％;其中稻瘟病抗性增强的材料7份,抗性变异率1.45％。实验表明:通过花粉管通道法或者孕穗期茎注射法将外源DNA导入水稻,均可以引起受体后代的变异,转移供体性状,培育单性状转移和优良性状集中的新品种。
DNA from upland rice Mongwang Gu, Yunnan O. officinalis and oats was introduced into paddy rice 'Southwest 175' by injection through pollen tube or by injecting exogenous DNA into the stem of rice in boot stage. Plants with varied characters which could be stably hereditable were obtained. Types of variation includedt growth period, shape of plant, content of protein and amino acid, resistance to rice blast and isoenzymes of esterase and peroxidase. Some of vaired characters were from donors and the other were new. 17 plants of different varied types were derived from 482 introduced offsprings, account for 3.5% in rate of variability, in which 7 plants increased in rice blast resistance and variable rate of resistance was 1.4%. The experiment indicates that both methods are able to cause variation of receptor progenies and transfer donors' characters. They are important ways for molecular breeding of paddy rice.
出处
《西南农业学报》
CSCD
1993年第2期21-25,共5页
Southwest China Journal of Agricultural Sciences
关键词
水稻
育种
变异
DNA
Rice Molecular breeding Exogenous DNA introduction Character variation
