摘要
目的研究猪改良深低温动物模型深低温不停循环 (DHNCA) 90min期间的脑保护作用。方法实验分假手术组 (n =4 ) ,37℃不予特殊处理 ;实验组 (n =6 ) ,18℃DHNCA 90min ,复温后观察生理指标变化、脑组织形态学和超微结构变化 ,分子生物学检测即早基因c fos和c jun的表达。 结果实验组光镜下可见到少量神经元形态学改变 ;电镜下线粒体形态基本正常 ,突触前膜有大量囊泡聚集 ;RT PCR提示c fos和c jun表达上调。结论猪改良动物模型在深低温脑保护研究中有效简便。DHN CA 90min对神经组织无明显损害 ,为深低温脑保护液辅助灌注提供了实验基础。
Objective To study the role of the modified pig animal model with deep hypothermia without circulatory arrest(DHNCA). Methods Pigs underwent cooling to 18 ℃ under modified cardiopulmonary bypass(CPB)without arrest. In sham-operated group (n=4),CPB was maintained in a state of normal temperature for 90 min. In test group animals (n=6) underwent 90 min of DHNCA at 18 ℃. The physiological conditions were followed up, and the morphological regions of the brain tissues were examined by photo and electron microscopy. The expressin of c-fos and c-jun was examined as well. Results No physiological derangement and little morphological change were observed in test group, and no change in sham-operated group. What's more, the expression of c-fos and c-jun was upregulated in test group. Conclusion The modified model is effective and simple in the study of DHCA brain protection. DHNCA technology had no significant damage to the neural tissue under 90 min arrest and could be lay the foundation for further experimentation.
出处
《上海第二医科大学学报》
CSCD
2004年第12期1011-1013,1020,共4页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市科委科技发展基金 (0 0 4 1 1 90 2 5)资助项目
