摘要
目的 :探讨原发性肝细胞癌 (HCC)组织中凋亡抑制蛋白bcl 2和bcl xl的表达与血管内皮生长因子 (VEGF)、碱性成纤维细胞生长因子 (bFGF)以及血管生成之间的关系。方法 :对经病理证实的肝细胞癌 38例共 4 0个癌灶进行分析 ,用免疫组化SP法检测癌组织中VEGF、bFGF、bcl 2和bcl xl的表达以及微血管密度 (MVD) ,分析它们之间的相互关系。结果 :VEGF、bFGF、bcl 2和bcl xl的阳性表达率分别为 77 5 % (31/4 0 )、75 % (30 /4 0 )、2 7 5 % (11/4 0 )和 5 0 % (2 0 /4 0 ) ,所有病灶的平均MVD为 39 3± 8 4 0。VEGF与bFGF、bcl 2与bcl xl之间明显相关 (P <0 0 1)。VEGF与bcl 2之间也存在一定的相关性 (P <0 0 5 )。在VEGF、bcl xl的阴性和阳性表达组MVD均具有显著的差异 (P <0 0 5 )。结论 :VEGF、bFGF与bcl 2、bcl xl之间具有一定的关系 ,VEGF和bcl
Objective:To explore the correlation of the apoptosis inhibitive proteins including bcl-2 and bcl-xl and the vascular endothelial growth factor (VEGF),basic fibroblast growth factor (bFGF) and angiogenesis in hepatocellular carcinoma (HCC).Methods:Thirty-eight cases (40 lesions) of HCC verified by histopathology were studied.The microvessel density (MVD) and the expressions of VEGF,bFGF,bcl-2 and bcl-xl were detected with immunohistochemical SP method,and the relationships among these results were analysed.Results:The positive rate of VEGF,bFGF,bcl-2 and bcl-xl was 77.5%(31/40),75%(30/40),27.5%(11/40) and 50%(20/40) in HCC respectively,and the mean MVD was 39.3±8.40.The correlations between VEGF and bFGF,and between bcl-2 and bcl-xl were found (P<0.01 respectively).The same result was also found between VEGF and bcl-2 (P<0.05).The MVD was higher in the positive expression group of bcl-xl or VEGF than in the negative expression ones (P<0.05 respectively).Conclusion:There are some correlation among the proteins as VEGF,bFGF,bcl-2 and bcl-xl,and the angiogenesis in HCC may be regulated by the expressions of VEGF and bcl-xl.
出处
《临床肿瘤学杂志》
CAS
2004年第6期565-567,共3页
Chinese Clinical Oncology
基金
山东省科技厅资助课题 (项目编号 1998BB1CCA1)
