摘要
目的 :探讨外源性生长激素对荷瘤小鼠化疗效果的影响。方法 :将 6 0只 6 15小鼠随机分为两群 ,一群用于观察肿瘤生长情况 (36只 ) ,一群用于观察生存期 (2 4只 )。每群动物经脾脏接种小鼠前胃癌细胞 (MFC)建立肝转移模型后 ,再随机分为 3组 :对照组、化疗组和联合组。术后 2周对照组每天皮下及腹腔注射生理盐水 ;化疗组每天腹腔注射 5 FU 2 0mg/kg ;联合组每天皮下注射重组人生长激素 (rhGH) 2IU/kg ,腹腔注射 5 FU 2 0mg/kg。各组均连续用药 7天。 4周时处死第一群动物 ,第二群小鼠待其自然死亡或观察满 70天处死。结果 :化疗组和联合组的肝转移瘤数目较少 ,肝重量和肝脏指数明显较低 (P <0 0 5 ) ,生存期也明显延长 (P <0 0 5 )。肿瘤细胞周期分析显示 ,联合组静止期 (G0 /G1)细胞百分比高于其它两组 (P<0 0 5 ) ,S期细胞百分比和增殖指数 (PI)均低于其它两组 (P <0 0 5 )。结论 :rhGH与 5 Fu合用抑制了肿瘤生长 ,可延长动物生存期 ,而对化疗效果无不良影响 ;rhGH可能通过加速细胞增殖周期 。
Objective:To study the effect of growth hormone on chemotherapy in tumor bearing mice. Methods: Hepatic metastases model were established in sixty 615 mice by splenic injection of proventriculus squamous carcinoma cell (MFC). Mice were divided randomly into 3 groups: control (NS),flurouracil (5-FU), and flurouracil plus rhGH(GH/5-FU). From 15 to 21days after surgery, the 5-FU group received daily i.c 5-FU(20mg/kg), the GH/5-FU group received daily s.c.recombinant human growth hormone (rhGH) injections (21IU/kg) and i.c.5-FU(20mg/kg), while the control group received daily s.c.and i.c.sterile saline injection at the same dosage. On day 28, some animals in each group were sacriced. The number of liver metastases and weight of liver were measured, and cell cycle of tumor DNA was determined by flow cytometry (FCM). The rest of animals in three groups were fed till death to observe survival time. Results:There were no differences in body weight among three groups (P>0.05). In the two groups received chemotherapy, the liver weight and the number of liver metastases were significantly lower and the lifespan were longer than those of the control group (P<0.05).DNA cell cycle analyses showed that the percentage of G1/G0-phase of hepatic tumors in GH/5-FU group was significantly increased comparing with that of the other two groups (P<0.05). The percentage of S-phase and proliferation index(PI) was decreased significantly in GH/5-FU group compared to the other groups (P<0.05) .Conclusion:rhGH combined with 5-FU inhibit tumor cells growth; make the animals’ lifespan longer, and there were no bad effects on chemotherapy. Cell cycle kinetics analyses showed that GH enhanced the sensitivity of tumor cell to cell cycle specific chemical anticarcinogenic drug, and reduced the proliferation activity of tumor cell by regulating tumor cells’proliferating cycle.
出处
《临床肿瘤学杂志》
CAS
2004年第6期574-577,共4页
Chinese Clinical Oncology
