摘要
目的 :建立高效液相色谱法测定调脂保肝颗粒中齐墩果酸的分析方法 .方法 :采用HypersilC18色谱分析柱(15 0mm× 4 .6mm ,5 μm) ,流动相为甲醇 水 冰乙酸 三乙胺(87∶13∶0 .0 4∶0 .0 2 ) ,检测波长 2 10nm ,流速 1.0mL·min -1.结果 :齐墩果酸在 18.6～ 130 .2mg·L -1范围内线性良好(r=0 .999) ,低、中、高 3种浓度平均回收率为 98.92 % ,10 2 .4 5 % ,99.5 7% (n =6 ) ,RSD分别为 1.6 1% ,1.32 % ,1.5 3% .结论 :本方法简便、快速、准确、灵敏、选择性强 。
AIM: To establish a HPLC method for the determination of oleanolic acid in Tiaozhibaogan granules. METHODS: Hypersil C18 column (150 mm×4.6 mm, 5 μm) was used, with the mobile phase being methanol water acetic acid triethylamine (87∶13∶0.04∶0.02), the flow rate 1.0 mL·min -1 and the detection wave length 210 nm. RESULTS: A good linear relationship was obtained between the peak area and the concentration of oleanolic acid from 18.6 to 130.2 mg·L -1 ( r = 0.999). For low, middle and high concentrations, the average recovery rate of oleanolic acid was 98.92%, 102.45% and 99.57% ( n = 6), and their RSD were 1.61%, 1.32% and 1.53%, respectively. CONCLUSION: Method, simple, rapid, sensitive, accurate and selective, can be used in controlling quality of oleanolic acid in Tiaozhibaogan granules.
出处
《第四军医大学学报》
北大核心
2003年第18期1674-1676,共3页
Journal of the Fourth Military Medical University
关键词
齐墩果酸
调脂保肝颗粒
色谱法
高效液相色谱法
oleanolic acid
Tiaozhibaogan granules
chromatography, high pressure liguid
