摘要
目的 探讨质膜Ca2 + - ATP酶异构体 2 (PMCA2 )基因多态性与噪声性听力损失的关系。方法 采用横断面流行病学研究方法 ,对 194名噪声暴露作业工人进行调查和听力测试 ,按听力学评价的结果将其分为听力损失组和听力正常组 ;用多聚酶链反应 限制性片断长度多态性 (PCR -RFLP)方法和等位基因特异扩增法 (ASA)检测其PMCA2基因上rs2 2 892 74和rs6790 64 0两个单核苷酸位点的多态性。结果 在 93名噪声性听力损失的工人中 ,rs2 2 892 74位点AA、AG和GG基因型的频率分别为16. 1%、40. 9%和 43. 0 % ,等位基因A和G的频率为 3 6 6%和 63 . 4% ;在 10 1名听力正常的工人中 ,其基因型频率分别为 15 8%(AA)、3 2 7% (AG)和 5 .1 5 % (GG) ,等位基因频率为 3 2. 2 % (A)和 67. 8% (G)。rs6790 64 0位点在噪声性听力损失组CC、CT和TT基因型的频率分别为 0、 82 8%和 17. 2 % ,等位基因C和T的频率为 41. 4%和 5 8 .6% ;在听力正常组的基因型频率分别为 1 0 %(CC)、 76. 2 % (CT)和 2 2 . 8% (TT) ;等位基因频率为 3 9. 1% (C)和 60. 9% (T)。两位点的基因型分布及其等位基因频率在噪声性听力损失组与听力正常组之间差异均无显著性 (P >0 . 0 5 )。采用多元Logistic回归对两组间年龄、性别、吸烟状况。
Objective To investigate the association of plasma membrane Ca 2+ -ATPase isomer 2 gene(PMCA2)polymorphisms with the development of noise-induced hearing loss(NIHL).Method Totally,194 workers exposed to occupational noise were studied cross-sectionally with hearing tests.According to the results of audiometry,they were divided into two groups,NIHL group and normal-hearing group.Polymorphisms of two single nucleotide loci rs2289274 and rs6790640 in the PMCA2 gene were determined by polymerase chain reaction followed by restriction fragment length polymorphism analysis(PCR-RFLP)and allele specific amplication analysis(ASA)in 93 workers with NIHL and 101 workers with normal hearing.Result Frequencies of genotypes AA,AG and GG in the rs2289274 locus were 16.1%,40.9% and 43.0%,respectively,in the NIHL group and 15.8%,32.7% and 51.5%,respectively,in the normal hearing group,and frequencies of alleles A and G in the same locus were 36.6% and 63.4%,respectively,in the NIHL group and 32.2% and 67.8%,respectively,in the normal hearing group.And,frequencies of genotypes CC,CT and TT in the rs6790640 locus were 0,82.8% and 17.2%,respectively,in the NIHL group and 1.0%,76.2% and 22.8%,respectively,in the normal hearing group,and frequencies of alleles C and T in the same locus were 41.4% and 58.6%,respectively,in the NIHL group and 39.1% and 60.9%,respectively,in the normal hearing group.There was no significant difference in frequencies of the genotypes and alleles of the rs2289274 and rs6790640 loci between NIHL group and normal hearing group(P>0.05).No significant higher risk for hearing loss was found in those with any of the genotypes of the two loci(P>0.05),adjusted for age,gender,smoking status,history of exposure to explosive noise and cumulative noise exposure(CNE)with multivariate logistic regression analysis.Conclusion It is suggested that genetic polymorphism of the rs2289274 and rs6790640 loci in the PMCA2 gene might not be a susceptible factor for NIHL.
出处
《中国工业医学杂志》
CAS
北大核心
2005年第1期19-22,共4页
Chinese Journal of Industrial Medicine
基金
国家自然科学基金资助项目 (编号 :30 371 2 0 4 )
