摘要
目的 分析肺癌组织GST-π基因表达和血浆水平的相关性及化疗前后血浆GST-π的水平变化。方法 运用RT-PCR方法对 60例经纤维支气管镜活检肺癌组织及 20例非癌良性肺组织进行GST-πmRNA定量检测,用化学比色法动态测定化疗前后血浆GST-π活力水平。结果 肺癌组织GST-πmRNA表达水平 (1 98±0 76)明显高于良性肺组织表达水平(1 02±0 45),表达阳性率分别为 45%、15%,GST-πmRNA表达与肿瘤组织病理类型有关(P<0 05),组织GST-πmRNA表达(1 98±0 76)与血浆GST-π活力(23 36±7 99)呈正相关(r=0 65,P<0 05),化疗前后血浆GST-π水平均明显高于对照组,化疗前后GST-π水平变化与疗效相关(P<0 05)。结论 肺癌组织恶变过程中GST-πmRNA基因表达及GST-π血浆水平增加,GST-π可以作为肺癌早期诊断的肿瘤标记物;化疗前后GST-π水平变化对临床化疗疗效判断有一定指导意义。
Objective To study the correlation between GST-π expression and plasma level in lung cancer and the plasma level of pre-or post-chemotherapy.Methods The tissue GST-π and the plasma GST-π level of 60 lung cancer patients and 20 noncancer patients were detected by reverse transcription-polymerase chain reaction(RT-PCR)and chemochromatometry respectively.Results GST-π gene expression in lung cancer tissues(1.98±0.76)was higher than that of the noncarcinomatous tissues(1.02±0.45).GST-π gene expression was correlated to pathological type(P<0.05).GST-π gene expression(1.98±0.76)was showed positive correlation with the plasma levels(23.36±7.99)(r=0.65,P<0.05).The plasma GST-π levels of pre-or post-chemotherapy was higher than the control subjects.Correlations were noted between the plasma levels of pre-or post-chemotherapy with therapy effect(P<0.05).Conclusion The genetic expression of GST-π and the plasma levels were increased in the cancerative course of lang cancer.GST-π can be a tumor marker in diagnosing lung cancer.The plasm level pre-or post-chemotherapy has a significant instructive value in estimating the curative effect.
出处
《中国厂矿医学》
2005年第1期5-7,共3页
Chinese Medicine of Factory and Mine
