摘要
目的探讨用荧光定量RT-PCR法检测胃癌患者腹腔洗出液中癌胚抗原mRNA(CEA-mRNA)含量的临床意义。方法采用荧光定量RT-PCR法检测98例胃癌患者及10例胃溃疡患者(对照组)手术中病灶切除前后腹腔洗出液中CEA-mRNA的含量,对不同病期及切除前后所测得的CEA-mRNA含量进行统计分析。结果本法可检测的下限约为10copies?ml,上限约为1.0×108copies?ml。检测所得荧光信号基线的循环数(CT值)与CEA-mRNA拷贝数对数值的相关系数为-1.0,错误率为0.0731。对照组腹腔洗出液CEA-mRNA的含量少于10copies?ml。胃癌患者腹腔洗出液中CEA-mRNA的含量病灶切除前为(0.0～5.62)×106copies?ml,几何均数为(172.11±63.07)copies?ml;切除后(256.4～4.67)×107copies?ml,几何均数为(606.32±76.21)copies?ml,两组的差别有显著性意义(P=0.008);切除前Ⅰa+Ⅰb期与Ⅱ期、Ⅱ期与Ⅲa+Ⅲb期、Ⅲa+Ⅲb期与Ⅳ期比较,其CEA-mRNA含量的差别均有显著性意义(P=0.007、0.015、0.027);同样,切除后各期比较,其差别也均有显著性意义(P=0.004、0.012、0.023)。切除前后未侵及浆膜与侵及浆膜者比较,其CEA-mRNA含量的差别有显著性意义(P=0.0008、0.0007);切除前后有淋巴结转移者与未转移者比较,其CEA-mRNA含量的差别有显著性意义(P=0.045、0.044);
Objective To apply the methods of real-time fluorescence-quantitative reverse transcriptases-polymerase chain reaction (real-time RT-PCR) for detection of CEA-mRNA levels in peritoneal lavage of gastric cancers and to investigate its clinical significance. Methods Peritoneal washes during operation was obtained from 98 patients with gastric carcinoma before and after tumor resections. CEA -mRNA levels were measured using real time PCR with fluorescent hybridization probes. Results The detection range of CEA- mRNA by real-time PCR 10copies/ml to 1.0×108 copies/ml, the correlation between CT value and log concentration of CEA-mRNA was -1.0, with an error rate of 0.0731. In 98 patients with gastric cancers the CEA-mRNA levels in peritoneal lavage were (172.11±63.07)copies/ml (before operation) and (606.32±76.21)copies/ml (after operation), P=0.008, while in the control group of 10 cases with gastric ulcer, the concentration of CEA -mRNA was lower than 10copies/ml. The CEA mRNA levels of gastric cancer patients with serosal invasion before or after operation were significantly higher than those without invasion(P<0.01). The CEA mRNA levels were significantly different among the different clinical stages(P<0.05). Conclusion Quantitative detection of CEA-mRNA levels in peritoneal washes is a practicable method with a good liner range, which can be used in clinical practice for patients with gastric cancer.
出处
《浙江医学》
CAS
2005年第1期15-18,共4页
Zhejiang Medical Journal
基金
浙江省医药卫生科学研究基金资助项目(2002A012)
