摘要
目的研究蛇床子和菟丝子及其复方制剂骨益补对维甲酸诱导大鼠骨质疏松的作用。方法70mg/kg维甲酸连续灌胃15d,诱导雄性大鼠形成骨质疏松模型,同时灌胃给药5周,以比色法测定其血清钙(sCa)、血清磷(sP)、骨碱性磷酸酶(bALP)和血清抗酒石酸酸性磷酸酶(sTRAP)的活性,竞争放射免疫法测定血清中骨钙素(sBGP)的含量;取干燥股骨及其骨灰测定其灰重系数,溶解后测定骨钙(bCa)和骨磷(bP)含量;双能X线(DEXA)测定股骨及腰椎的骨密度;三点弯曲试验法测定骨生物力学;扫描电镜进行骨小梁形态计量学分析。结果蛇床子和菟丝子以及复方骨益补能降低sTRAP和sBGP水平,增加股骨骨密度及bCa、bP含量,改善股骨抗弯抗拉能力,改善骨小梁形态,显著增加骨小梁平均宽度(MTT)与骨小梁面积百分比(TS%)。结论蛇床子和菟丝子以及复方骨益补对维甲酸诱导大鼠骨质疏松症具有防治作用。
AIM:To study the effects of GYB (Ext.of Fructus cnidii and Semen cuscutae)on osteoporosis rats induced by retinoic acid.METHOD:The osteoporosis of rats was induced by administering 70 mg/kg retinoic acid for 15 days.Having been fed with GYB,at the dose of 100,200,400 mg/kg·d,extracts of Fructus cnidii and Semen cuscutae,at the dose of 200 mg/kg·d,to the rats continuously for 5 weeks,the concentrations of serum calcium(s-Ca),serum phosphorus(s-P),tartrate resistant acid phosphatase in serum(s-TRAP)and alkaline phosphatease in bone(b-ALP)were measured by colorimetric method.The concentration of osteocalcin in serum(s-BGP)was measured by competitive radio immunological assay.The content of bone calcium(b-Ca),and bone phosphorus(b-P)in femur were determined by colorimetric method.Bone mineral density(BMD)of femur and lumbar was determined by Dual Energy X-ray Absorbtiometry(DEXA).Biomechanical characters of femur were measured by three point bending test.Histomorphometry of bone trabecula was analysed by scanning electron microscope.RESULT:The BMD of femur and the content of b-Ca,b-P were increased.The levels of s-TRAP and s-BGP were decreased.The resistance of bending and compression of femur was enhanced.The form of bone trabecula was bettered,MTT and trabecula square percentage(TS%)were increased obviously too.CONCLUSION:Fructus cnidii,Semen cuscutae and GYB have antiosteoporotic effects in rats induced by retinoic acid.
出处
《中国天然药物》
SCIE
CAS
CSCD
2005年第1期56-59,共4页
