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组胺受体拮抗剂对静脉“桥”保护作用的研究 被引量:5

A Study of the Preservation Effect on Vein Graft induced by Histamine Receptor antagonist
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摘要 目的探讨含组胺H1受体拮抗剂的保存液对静脉桥的保护作用。方法采集40例冠状动脉搭桥术(CABG)患者的大隐静脉(GSV),分别保存在常规罂粟碱林格液(Pap组)和含组胺H1受体拮抗剂——酮替芬的罂粟碱林格液(Ket组)中1小时。采用①免疫组化法检测血管内皮细胞(VEC)黏附分子(AM)的表达(IOD值),包括血管细胞黏附分子-1(VCAM-1)、细胞间黏附分子-1(ICAM-1)、P-选择素(P-selectin)、E-选择素(E-selectin);②硝酸还原酶法比较单位面积VEC一氧化氮(NO)的合成释放量;③每例各取两段GSV分别置于30%的小牛血清培养液(A组)和含酮替芬(100μg/ml)的同样小牛血清培养液(B组)中,体外培养14天后,采用抗5-Brdu免疫组化染色法计算平滑肌细胞增殖指数。结果①Ket组VCAM-1和ICAM-1的表达明显低于Pap组(P<0.01)而P-selectin和E-selectin的表达无统计学意义(P>0.05);②B组平滑肌细胞增殖指数明显低于A组(P<0.01);③单位面积VEC的NO合成释放量则明显高于Pap组(P<0.01)。结论含酮替芬的新型保存液能有效降低VEC表面VCAM-1、ICAM-1的表达,抑制内膜增生并且增加NO的合成释放,理论上提示了H1受体拮抗剂能够抑制静脉“桥”发生粥样硬化,延缓其再狭窄进而提高“桥”的远期通畅率。 Objective To study a preservation solution containing histamine H1 receptor antagonist and its protective effect on vein graft. Methods The residual parts of greater saphenous vein (GSV) taken from 40 patients underwent coronary artery bypass surgery (CABG) were entered the study. Each segment was preserved in Ketotifen Papaverine Ringers Solution (Ket group) and routine Papaverine Ringers Solution (Pap group) for one hour respectively,the expression of AM(VCAM-1, ICAM-1, P-selectin and E-selectin) were measured; and the dose of nitric oxid (NO) synthesized and released per unit area were examined; another two segments taken from each GSV were incubated in 30% calf serum(group A) and in the same serum but containing ketotifen(100μg/ml)(group B) respectively for fourteen days, smooth muscle cell(SMC) proliferation index was measured. Results The expression of VCAM-1 and ICAM-1 in Ket group was obviously lower than that in Pap group(P<0.01), but the expression of P-selectin and E-selectin made no difference in two groups ; the dose of NO synthesized and released per unit area was much higher in Ket group than that in Pap group (P<0.01);the SMC proliferation index in group B was obviously lower than that in group A (P<0.01).Conclusions This new preservation solution containing ketotifen may maximally reduce the expression of VCAM-1 and ICAM-1 on VEC, restrain the proliferation of endothelium and increase NO synthesis and release, We can conclude that H1-receptor antagonist may prevent the atherosclerosis and restenosis of vein graft theoretically, accordingly may improve the graft's long-term patency.
出处 《中国心血管病研究》 CAS 2005年第2期103-105,共3页 Chinese Journal of Cardiovascular Research
关键词 冠状动脉旁路移植术 酮替芬 黏附分子 隐静脉 内膜增生 Coronary artery bypass grafting Ketotifen Adhesion molecules Saphenous vein Endothelium proliferation
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