摘要
目的 :制备诊断丁型肝炎病毒 (HDV)cDNA基因芯片探针。 方法 :针对HDV病毒基因保守区域设计聚合酶链反应 (PCR)引物 ,纯化PCR扩增产物克隆 ,并测序鉴定。 结果 :序列分析表明 ,所扩增的片段均属于HDV特异基因。 结论 :利用PCR扩增产物制备基因芯片探针是一种快速、简便、实用的方法。
Objectives:To preparing the probes of cDNA microarray in detection of the Hepatitis D virus. Methods:The specific primers of PCR were designed accordion to the conserved region of HDV. Results: Sequences were aligned , and the results showed that the products of PCR amplification were the specific gene fragments of HDV. Conclusions:Using PCR amplification products to prepare gene chip probe was a quickly, simple effective method.
出处
《医学研究生学报》
CAS
2003年第11期803-804,810,I011,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目 (批准号 :3 9880 0 3 2 )
广州市重大科技攻关项目 (批准号 :99 Z 0 2 2 0 1)
