摘要
目的 观察 17β 雌二醇 (17β E2 )和ICI 182 780对人成骨肉瘤MG6 3细胞株表达护骨素(OPG)、OPG配体 (OPGL)及其相关因子 [TRAIL、巨噬细胞集落刺激因子 (M CSF)、转化生长因子 β(TGFβ) ]的影响。方法 用逆转录PCR(RT PCR)法检测基因表达情况 ,用Westernblot分析法检测蛋白表达情况。结果 (1) 17β E2 能上调MG6 3细胞中OPG及TGFβ的表达 ,下调OPGL、M CSF及TRAIL的表达。 (2 )ICI 182 780对被检测基因有不同的调节活性。结论 雌激素缺乏可能导致成骨细胞中OPG、TGFβ的表达减少 ,而解除了对OPGL、M CSF和TRAIL等细胞因子的抑制作用 ,使破骨细胞的数量和活性增加 ,骨吸收增强和骨量丢失 ,这可能是绝经后骨质疏松症的一个重要的发病机制 ;ICI 182 780并非纯雌激素受体拮抗剂 ,而属于选择性雌激素受体调节剂。
Objective To observe the regulative effects of 17β-estradiol (17β-E 2) and ICI 182780 on the expression of osteoprotegerin(OPG),the ligand of osteoprotegerin (OPGL) and the related cytokines [macrophage colony-stimulating factor (M-CSF),TRAIL and transforming growth factor β(TGFβ)] in MG63 cells.Methods The expression of OPG,OPGL,M-CSF,TRAIL and TGFβ mRNA was examined by reverse transcriptase(RT)-PCR. The expression of OPG and OPGL protein was measured with Western blot. Results (1)17β-E 2 up-regulated the expression of OPG and TGFβ but down-regulated OPGL,M-CSF and TRAIL expression in MG63 cells. (2)ICI 182780 showed varied transactivating capability when regulating the expression of OPG、OPGL、TRAIL、M-CSF and TGFβ genes in MG63 cells. Conclusions (1) One of the key pathogenetic factors of postmenopausal osteoporosis is that estrogen deficiency leads to the decreasing expression of OPG and TGFβ but the increasing expression of some bone-resorbing cytokines such as OPGL、M-CSF and TRAIL in osteoblasts,then stimulation of osteoclast differentiation and activity which potentiates bone resorption and bone loss;(2) ICI 182780 shows partial transactivating activity in osteoblasts, so it should belong to a kind of selective estrogen receptor modulators.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2003年第11期800-803,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金资助项目 ( 3 9970 3 47)
