摘要
建立测定保健食品中微囊化β-胡萝卜素的分光光度法, 采用正交设计确定了提取β-胡萝卜素的最佳条件,样品 经酶解,用氯仿提取后,转至环已烷溶剂中,在454nm波 长下测吸收值,通过E值计算其含量。方法的回收率为 92 5％-96.6％.相对标准偏差为2.4％-2.6％。结果表明,该 方法具有准确、简便的特点。
A spectrophotometric method to measure the content of the microencapsulated (3 -carotene in health foods has been established. The most suitable condition of extracting 3 -carotene were found by orthogonal tests. The sample was hydrolyzed by enzymes, extracted with chloroform and transferred into cyclo-hexane. The absorption value was measured at 454nm. The content was calculated based on the value of absorption coefficient E. The recovery rate of the method was 92.5%-96.6%, the relative standard deviation was 2.4%-2.6%. The result shows that method has the accurate and simple characteristics.
出处
《食品工业科技》
CAS
CSCD
北大核心
2005年第2期187-188,共2页
Science and Technology of Food Industry
