摘要
核酸分析是生命科学研究中最重要的技术之一。目前主要是应用核酸内源紫外吸收光谱的紫外分光光度法和基于荧光探针分子与核酸相互作用的荧光分光光度法。紫外分光光度法灵敏度低 ,荧光分光光度法试剂昂贵 ,有毒性。近年来 ,共振光散射技术在核酸分析中的应用得到了迅速的发展。核酸的共振光散射分析方法可以用普通的荧光分光光度计进行测定 ,应用安全、便宜的试剂获得很高的灵敏度。简要介绍了共振光散射分析的基本原理 ,并对近年来利用共振光散射技术分析核酸的研究进行了评述。内容主要包括利用有机染料分子作为核酸的共振光散射探针的分析方法 ;基于阳离子表面活性剂、金属离子及其络合物以及药物与核酸相互作用的分析方法 ;核酸形成大粒子的散射分析方法。
Nucleic acid analysis is one of the most important techniques for the study in biotic field. The spectrophotometry using inherent ultraviolet absorption spectrum of nucleic acids and the spectrofluorimetry based on the interaction of nucleic acids and fluorescence probes were usually used for nucleic acid analysis. However, the spectrophotometry was severely limited by its low sensitivity, and the spectrofluorimetry suffered from the disadvantages of high cost and toxic reagents. Recently, resonance light scattering has emerged as a new nucleic acid analysis technique. The resonance light scattering analysis is becoming increasingly important because high sensitivity can be obtained with a common spectrofluorimeter by using inexpensive and safe reagents. In this paper, the principle of resonance light scattering was briefly stated and the recent advances of the nucleic acid analysis by resonance light scattering were reviewed with 57 references. The resonance light scattering probes for nucleic acid analysis were divided into four groups: organic dyes; cation surfactants; metal ions and their complexes; and drugs. The light scattering analysis for nucleic acids based on the formation of large particles was also stated.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第12期1676-1680,共5页
Spectroscopy and Spectral Analysis
基金
河北省自然科学基金资助
