摘要
本研究对日本血吸虫感染小鼠脾细胞体外在SEA与ConA诱导下产生的IL 5和IL 10 2种细胞因子从mRNA转录水平进行研究 ,以探索这 2种细胞因子mRNA转录水平的动态变化与肉芽肿形成与调节的可能性。日本血吸虫尾蚴感染雄性BALB C小鼠后 ,分别于感染后 3周、 5周、 8周、 10周和 12周取小鼠脾脏 ,制备脾细胞单细胞悬液并于含ConA或SEA的培养基中培养后提取脾细胞总RNA。RT PCR及凝胶分析法被用来分析总RNA样品中IL 5及IL 10mRNA转录水平。研究结果显示 ,未感染和感染 3周小鼠脾细胞均无IL 5和IL 10的表达 ,感染后第 5周的小鼠脾细胞出现IL 5特异性条带 ,感染后第 8周IL 5表达明显增强 ,感染后第 10周IL 5mRNA转录水平下降 ,感染后第 12周未能检测到IL 5表达 ,表明IL 5表达的动态变化与肉芽肿的形成、发展及调节相平行。IL 10mRNA转录也于感染后第 5周出现 ,但感染后第 8周、第 10周、第 12周与第 5周相比 ,IL 10的表达水平并无明显改变 ,显示IL 10mRNA转录水平并未随着肉芽肿的调节而变化。本研究结果提示IL 5可能在肉芽肿形成和调节过程中可能起重要作用 ;而IL 10可能并未直接参与肉芽肿的调节 ,但其转录可能与防止宿主过度肉芽肿炎症反应有关。
In the present study,IL 5 and IL 10 mRNA levels were investigated in Schistosomiasis japonica in order to find out how relevant the dynamics of the gene expressions of the two cytokines to granulomatous inflammation.The spleens of S.japonicum infected BALB/c mice were removed at 0,3,5,8,10 and 12 weeks postinfection.The splenic cells were extracted for total RNA immediately after 4 hours incubation in the predence of ConA or SEA.The extracted RNA was analyzed for IL 5 and IL 10 mRNAs by RT PCR.It was found that the expressions of both IL 5 and IL 10 could not be detected in SEA or ConA treated spleen cells from both uninfected and 3 week infected mice.IL 5 mRNA could be found obviously expressed at 5 week infection,significantly increased at 8 week infection,and sharply declined at 10 week infection.However,no IL 5 expression was induced at 12 week infection.The observation revealed that the dynamic of IL 5 gene expression paralleled to the granuloma formation and modulation in S.japonicum infected BALB/c mice.In contrast to IL 5 mRNA expression,no apparent alternation in the level of IL 10 transcription was discovered from 5 to 12 week infection indicating IL 10 transcripiton level was not correspondently regulated when the infection proceeded,These results suggest that IL 5 may play an important role in the modulation of granuloma formation in S.japonica,it is also postulated IL 10 might be helpful to prevent severe immunopathology from excess granulomatous inflammation by a negative feedback regulation of the immune response.
出处
《寄生虫与医学昆虫学报》
CAS
2001年第4期204-211,共8页
Acta Parasitologica et Medica Entomologica Sinica
基金
UNDP WorldBank WHOSpecialProgrammeforResearchandTraininginTropicalDiseases资助项目
