摘要
采用高效毛细管电泳的胶束电动毛细管色谱的分离模式(MEKC),对泽泻的乙醇(95%)提取物进行分离研究。以十二烷基硫酸钠(SDS)胶束为准固定相,硼砂为缓冲体系,甲醇为有机添加剂,考察了SDS浓度、硼砂浓度、pH、甲醇用量、电泳电压、电泳温度等对分离的影响。结果表明:硼砂缓冲体系为运行缓冲溶液(pH:9.18～9.20)、硼砂浓度30mmol/L、SDS浓度45mmol/L、甲醇体积分数30%,分离电压25kV、分离温度25℃时分离效果最好。在此条件下,得到分离度和重现性均较好的泽泻乙醇提取物的HPCE色谱图。
The separation of the compounds extracted from rhizoma of Alisma orientalis (Sam.) Juzep using 95% ethanol by micellar electrokinetic capillary chromatography (MKCC) with sodium dodecyl sulphate (SDS) as the micellar phase, borax as a buffer and methanol as an organic additive was studied, which established a fingerprint technique for the rhizoma of Alisma orientalis. The effects of SDS micellar and borax concentration, pH buffer, methanol volume fraction, applied voltage and temperature on the separation were investigated by the orthogonal test using chromatography optimization function (COF) as the evaluation index. It was found that the optimal separation conditions of these components might be achieved by using a fused silica capillary with 60 cm long and 75 μm ID, in which 30 mmol/L borax buffer solution (pH 9.20) containing 45 mmol/L SDS and 30% methanol was filled under the voltage of 25 kV and a temperature of 25 ℃. HPCE fingerprint of the rhizome of Alisma orientalis with perfect separating size and repeatability could be expected. Thus, the method provides a basic information for the whole quantity control of the rhizoma of Alisma orientalis in the products.
出处
《分析试验室》
CAS
CSCD
北大核心
2005年第1期8-10,共3页
Chinese Journal of Analysis Laboratory
关键词
泽泻
胶束电动毛细管色谱
分离
Alisma orientalis
Micellar electrokinetic capillary chromatography
Separation
