摘要
首次应用Mu转座重组技术研究铜绿假单胞菌色素合成与调控的机制。通过一系列的表型筛选 ,得到 8株色素合成能力改变的突变子。经基因克隆、核苷酸测序研究 ,证明转座子分别插入到hmgA、ptsP、sucC、phzS、phzF1五个基因中。hmgA基因转座失活导致酪氨酸分解代谢中间产物尿黑酸的积累 ,后四种情况转座突变显著地影响了铜绿假单胞菌最重要的色素 绿脓素 (pyocyanin)的合成 ,其中PhzS和phzF1是绿脓素合成过程中的结构基因 ,ptsP基因是 1个磷酸转移酶系统的重要组分 ,sucC基因的产物是三羧酸循环中的琥珀酰辅酶A合成酶 。
Mu transposition recombination technique was firstly used as a mutagenesis tool to explore a cluster of genes involved in biosynthesis and regulation of pigments in P. aeruginosa. Eight pigment mutants were screened and identified. Gene cloning and sequencing of the region flanking the insertion revealed that the genes hmgA、ptsP、sucC、phzS、phzF1 were disrupted with mini-Mu respectively. Among them, gene hmgA is involved in the degradation of tyrosine, others affect the metabolism of pyocyanin which is the most important pigment of P. aeruginosa. Both gene phzS and gene phzF1 have been experimentally demonstrated to participate in pyocyanin synthesis. Gene ptsP belongs to a phosphotransferase system and gene sucC encodes succinyl-CoA synthetase beta chain. This is the first report that gene ptsP and sucC may be involved in the regulation of the biosynthesis of pyocyanin.
出处
《微生物学报》
CAS
CSCD
北大核心
2005年第1期14-18,i003,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金 ( 3 0 2 70 0 75 )~~
