摘要
目的研究胃肠道间质瘤(GISTs)临床和病理形态特点及免疫组织化学在诊断中的价值,讨论肿瘤的组织来源、治疗和预后。方法对51例GISTs进行光镜观察,用SP免疫组织化学方法检测CD117(c-kit)、CD34、波形蛋白、actin、s-100等抗原标记物在肿瘤中的表达情况。结果51例GISTs,其中40例以梭形细胞为主;17例伴有上皮样细胞,4例单纯由上皮样细胞组成。免疫组织化学显示肿瘤组织中抗原标记物表达阳性率分别为CD11795.0%(48/51),CD3480.0%(41/51)。结论GISTs是消化道最常见的间叶性肿瘤,光镜形态与真性肌源性和神经源性肿瘤极为相似,免疫标记物配合使用可对其做作出正确诊断,GISTs可能起源于多潜能的间质干细胞。
Objective:To investigate the clinical and pathologic morphological features,immunohistochemical speciality of the gastrointestinal stromal tumors(GISTs) and their histogenesis,treatment and prognosis as well.Methods:The morphologic characteristics of GISTs were studied in 51 cases using light microscopy.The expression of c-kit(CDll7),CD34,vimentin,actin,S-100 were detected in all the cases with SP immunohistochemistry staining.Results:Among 51 cases of GISTs,40 were spindle cell type,4 epithelioid cell type and 17 mixturetype.Almost all of the stromal tumors were strongly positive for vimentin(99%),48 out of 51 tumors positive for CD117 (c-kit)(95.0 %) and 41 positive for CD34(78.5%).Some cases also expressed actin,S-100.Conclusion:GISTs are the most frequent mesenchymal tumor seen in the gastrointestinal tract.Under light microscope,the morphology of stromal tumors looks sometimes like that of a leiomyoma and Schwannoma.Application of immunohistochemical markers (particularly CD117 and CD34)is considered necessary for the differential diagnosis.GISTs may originate from the pluripotential stem cell of the interstium.
出处
《重庆医科大学学报》
CAS
CSCD
2005年第1期98-101,共4页
Journal of Chongqing Medical University
