摘要
试验采用 8个耐酸铝明显不同的大豆品种 ,研究了在铝胁迫下大豆有机酸分泌及其与耐性的关系。同时也比较了大豆铝敏感品种LJ和小麦耐铝品种Atlas在铝胁迫下有机酸分泌的差异。大豆幼苗在含 10 μMAlCl3的 0 .5mMCaCl2 溶液中进行耐酸铝性筛选。结果表明 ,大豆品种在耐酸铝方面存在明显的不同。大豆在 5 0 μMAlCl3铝胁迫下可以分泌大量的柠檬酸 ,但柠檬酸分泌量与大豆的耐酸铝性没有正相关性。经过铝处理一天的大豆 ,恢复一天后再用铝处理 ,多数品种分泌的柠檬酸明显减少 ,特别是敏感品种柠檬酸分泌大幅减少 ,但柠檬酸分泌量与耐酸铝性也没有相关性。进一步试验表明 ,大豆铝敏感品种LJ分泌的柠檬酸明显高于小麦耐性品种Atlas分泌的有机酸总量 (柠檬酸和苹果酸 )。另外 ,大豆主根的铝积累与耐铝性及柠檬酸分泌量没有相关性。因此 ,试验结果表明 ,铝诱导的有机酸分泌量及根尖的铝含量不能作为筛选大豆耐酸铝种质的筛选指标。
The varietal differences in Al-induced citrate exudation and its relation to Al tolerance were investigated, and a comparative study of differences in organic acid secretion between Al-sensitive soybean LJ and Al-tolerant wheat Atlas was conducted. Soybeans were exposed to the 0.5 mM Ca solution containing 0 and 10 μM AlCl 3 to identify differences in Al tolerance. Based on relative root elongation (% of control), soybeans exhibited a range of difference in Al tolerance. Citrate secretion under 50 mM AlCl 3 (24 h) stress differed among the 8 varieties used, but no relation between the Al tolerance and Al-induced citrate secretion was observed. Al-induced citrate secretions from most of the varieties was greatly reduced by pre-treatment with 50 AlCl 3 mM for 24 h. Sensitive soybean LJ exuded more organic acid (citrate only) than did Al-tolerant wheat Atlas, which secreted both citrate and malate. Al accumulation in root tips was not related to Al tolerance and Al-induced citrate secretion. Thus, this experiment suggests that Al-induced citrate exudation and Al accumulation in root tips can not be used as standards for screening Al-tolerant soybeans.
出处
《大豆科学》
CAS
CSCD
北大核心
2003年第3期218-222,共5页
Soybean Science
基金
国家自然科学基金资助项目 (批准号 :3 970 0 86)
农业部 948资助项目 ( 9910 2 3 )
