摘要
目的 探讨反应停对亲代与耐顺铂人肺腺癌细胞系A549DDP碱性成纤维细胞生长因子 (bFGF)表达的影响及克服肺腺癌顺铂耐药的作用。方法 采用逆转录 PCR和免疫细胞化学方法分别检测反应停处理前、后A549与A549DDP细胞bFGFmRNA与蛋白表达水平。结果 治疗浓度(6μg/ml) 反应停处理A549与A549DDP细胞后bFGFmRNA表达水平较处理前显著降低 ,蛋白表达显著减弱 ,蛋白表达与其相应mRNA表达呈显著正相关 ;不同浓度反应停处理后第 5d ,A549与A549DDP细胞bFGFmRNA表达水平存在显著性差异 ,蛋白表达均为阴性。结论 反应停呈剂量依赖性显著下调A549与A549DDP细胞bFGFmRNA表达 。
Objective To elucidate the effects of thalidomide on the expressions of basic fibroblast growth factor(bFGF) in parental and cisplatin resistant human lung adenocarcinoma cell line A 549 and A 549 DDP Methods RT PCR and lmmunohistochemistry were used to detect the bFGF mRNA and protein expression in A 549 and A 549 DDP Results bFGF mRNA expression levels and protein expressions in A 549 and A 549 DDP were all significant reduced after treatment of thalidomide (6 μg/ml)(all P <0 001).There existed significant differences in A 549 and A 549 DDP among different concentrations of thalidomide on the 5 th day The bFGF protein expression level was consistent with the mRNA expression levels in A 549 and A 549 DDP Conclusion Thalidomide down regulated bFGF expression significantly in A 549 and A 549 DDP cell lines in a dose dependent manner It was suggested that thalidomide not only inhibit tumor angiogenesis but also induce apoptosis
出处
《中国呼吸与危重监护杂志》
CAS
2003年第1期40-43,共4页
Chinese Journal of Respiratory and Critical Care Medicine
基金
国家"九五"课题攻关资助项目 ( 96 90 6 0 1 2 3)
北京市自然科学基金资助项目 ( 7992 0 0 5)
