摘要
目的：研究人骨肉瘤细胞凋亡及P53、PCNA的表达情况，并初步探讨其临床及生物学意义。方法：采用原位DNA末端标记法及免疫组化方法检测了39例骨肉瘤中细胞凋亡及P53、PCNA的表达情况，并统计其阳性反应率。结果：本组病例中细胞凋亡阳性率61．5％，P53呈阳性反应29例，阳性率74．3％，PCNA呈阳性反应34例，阳性率87．2％。结论：骨肉瘤组织中有P53基因和PCNA的高表达，与肿瘤的预后相关。在骨肉瘤中细胞凋亡是一种自然现象，其发生情况各不相同，研究初步提示了其与预后相关，并应与P53蛋白和PCNA的表达相结合而综合判断。
Objective:To determine the expression of apoptosis,P53 and PCNA in osteosarcoma and to evaluate preliminary significance in clinic and biology. Methods: A series of 39 osteosarcoma was analyzed for the expression of apoposis by 3end - labeling method of DNA in tissue section and the expression of P53 and PCNA by immunohistochemical techique. The indexes of apoptosis, P53 and PCNA were respectively the percentages of apoptosis cells or P53 and PCNA positive cells. Results: Apoposis occurred in 61. 5% of osteosarcoma,the positive rate of P53 was 74. 3% (29/39), and PCNA was 87. 2% (34/39). Conclusion:There were P53 and PCNA high expression in osteosarcoma and high expression associated with prognosis. The apoptosis was a spontaneous phenomenon in osteosarcoma and the expression was different from each other. The apoptosis associated with prognosis and it could be better to judge with the expression of P53 and PCNA.
出处
《黑龙江医学》
1999年第10期1-2,共2页
Heilongjiang Medical Journal
基金
黑龙江省教委科研项目基金
