摘要
孕妇感染弓形虫是胎儿先天性畸形的危险因素。本文运用被称之为ＤＮＡ聚合酶链反应的体外基因扩增技术检测３０例先天性畸形儿尸检石蜡包埋心肌组织弓形虫。结果表明，３０例先天性畸形儿石蜡心肌组织切片经体外扩增后出现阳性条带者１３例，而对照组（１０例）无一例阳性（０．０５＞Ｐ＞０．０１）。研究结果进一步支持弓形虫的感染与胎儿先天性畸形有密切关系，本文还讨论了聚合酶链反应技术与其它最新技术比较，聚合酶链反应技术具有微量、特异性强、敏感度高、反应周期短、操作简便，对长期保存并已消失感染性的样品也有效等优点，适应于临床推广。
Infection of pregnant women with Toxoplasma gondii is a risk factor of fetal congenital malformation.Toxoplasma gondii DNA in paraffinembedded thin-tissue sections was measured by the in vitro gene amplifi cation technique known as the polymerase chain reaction(PCR).The results revealed that the paraffinembedded myocardium tissue sections Toxoplasma gondii DNA sequences after the in vitro gene amplification showed 13 cases positivity bends in 30 deformity fetus,no case positivity bend in 10 controls(0.05>p>0.01).The study offers further support of the view that infection of Toxoplasma gondii was associated with fetal congenital abnormality.The PCR was evaluated for diagnosis of fetal infection Toxoplasma gondii and compared with the current methods.Therefor e the method described in the paper in a microdetermination,specific,highly sensitive and rapid conventional.
出处
《中国优生与遗传杂志》
1994年第1期18-20,共3页
Chinese Journal of Birth Health & Heredity
