摘要
用4%纤维素酶RS,2%半纤维素酶和1%果胶酶Y-23及多聚半乳糖醛酸酶的混合酶液,从蜂斗菜(Petasites japonicus)叶片分离出高产率的原生质体。原生质体培养在无机盐为1/4 MS、维生素为1/2MS、附加NAA2 mg/L、BA 0.2—0.5mg/L的甘露醇0.5mol/L、蔗糖10 g/L的液体培养基里,分裂旺盛。形成愈伤组织后经芽的诱导,生根培养获得了再生植株。
Protoplasts were isolated by enzyme digestion from leaf of Japanese butterbur (Petasites japonicus). The enzyme incubation mixture consisted of 4% (W/V)cellulase RS, 2% (W/V) hemicellulase, 1% (W/V) pectinase-dissolved Y-23 and polygalacturonase in a solution of 0. 5 mol/L mannitol at pH 5. 7 . In the basic medium of 1/4 MS inorganic salts and 1/2 MS vitamins supplemented with 2 mg/L NAA,0. 2-0. 5mg/L BA, 0. 5 mol/L mannitol and 10 g/L sucrose, the cells divided luxuriantly. Regenerated plantlets were formed from callus after bud induction and root initiation.
关键词
蜂斗菜
原生质体培养
植株再生
Petasites japonicus
Protoplast culture
Plantlet regeneration
