摘要
从湖南、广东两省六地采集卫氏并殖吸虫和斯氏狸殖吸虫,分别制备各地虫体基因组DNA,用 BamHI、HaeⅢ及 HindⅢ 3种限制性内切酶消化,琼酯糖凝胶电泳后,比较酶切片段长度。结果显示两种虫种间重复 DNA序列带型有明显的差异,而同种虫种不同地区虫体的重复 DNA序列带型则多数相同或完全相同。
The genomic DNA of the two lung flukes (P. westermani and P. skrjabini)from six areas was digested separately by three restriction endonucleases BamHI,Hae Ⅲ and Hind Ⅲ (followed by agarose gel electrophoresis. Repetitive DNA were visusalized as distinct bands in stained gels. Restriction fragment length differences were detected between the two species. The majority of bands of repetitive DNA were similar with minor differences within the same species collected from different areas,upon digestion with certain restriction endonucleases.The results demonstrated that the technique for demonstrating the band pattern of repetitive DNA with three restriction endonucleases can be used for the identification of P. westermani and P. skrjabini and the geographic relationship of the same species from different areas (Figs. 1 - 3).
出处
《中国寄生虫学与寄生虫病杂志》
CSCD
北大核心
1993年第4期279-281,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
卫氏并殖吸虫
斯氏狸殖吸虫
DNA
Paragonimus westermani
Pagumogonimus skrjabini
repetitive DNA sequence
restriction endonuclease.
