摘要
目的：建立检测结核抗体的酶联免疫吸附试验，并探讨方法的最佳测定条件。方法：采用结核分枝杆菌38—KDa基因重组抗原包被固相载体进行酶联免疫测定。结果：170份血清结核抗体测定，敏感性为74．0％，特异性为95．7％；Omega试剂的敏感性为68．0％，特异性为97．1％。两种38－KDa重组抗原的测定结果差异无显著性（P＞0．05）。结论：38一KDa。抗原性能达到或接近Omega产品.本法灵敏、特异，可作为结核病的辅助诊断方法之一。
0bjective: Establish enzyme - immunoassay for the detection of tubercle antibodies and describeits optimal conditions. Methods:With recombinant 38 - KDa antigen of M. tuberculosis coating soid carrier.Results: The sensitivity and specificity of this method was 74. O % and 95. 7 %, while the Omega reagent was68. 0 % and 97. 1 % respectively by detection of l70 sera samples. There was no significantly differences in re-suIts by two reagents (P >0. O5). Conclusion: According to its sensitity and specificity value, this method canserve as supplementary method f0r the diagnosis of tuberculosis.
出处
《上海预防医学》
CAS
1998年第10期450-452,共3页
Shanghai Journal of Preventive Medicine
关键词
结核分枝杆菌
基因工程
免症测定
抗体
Antigen
Mycobacterium tuberculosis
Genetic engineering
Immunoassay
Antibody
