摘要
目的：研究不同分子生物学方法检测乙型肝炎病毒（HBV）DNA的敏感性及影响因素。方法：用饱和酚抽提和血清蛋白变性后聚合酶链反应（PCR－1和PCR－2）检测48例慢性肝病患者的HBVDNA，并与分子斑点杂交法（Dot－H）检测结果进行比较。结果：Dot－H、PCR－1和PCR－2法对HBVDNA的检出率分别为70．83％、87．50％和91．67％，PCR－1和PCR－2中有17．02％表现为交叉阳性，慢性肝炎患者的检出率高于肝硬化患者，HBeAg阳性者高于其它HBV血清标志物（HBVm）阳性者。结论：PCR检测HBVDNA的敏感性高于Dot－H，所用PCR包含的多种引物可以提高HBVDNA的检出率。
Objective To study sensitivity and influence factor of the determination of hepatitis B virus (HBV) DNA by different methods of molecular biology. Methods The polymerase chain reaction (PCR) assay was applied to detect HBV DNA in sera of 48 patients with chronic liver diseases. The samples were respectively perpared with procedures of the phenol extraction (PCR-1 ) and the rapid method of protein denaturation(PCR-2). The results of the two methods were compared with those of dot hybridization(Dot- H) analysis. Results The detective rate of HBV DNA in the Dot-H, PCR-1 and PCR-2 were 70. 83%, 87. 50% and 91. 67%. The cross positive rate of PCR-1 and PCR-2 was 17. 02%. The detection rate of HBV DNA in the patients with chronic hepatitis was higher than that with liver cirrhosis and it was higher in the patients with positive HBeAg than that with other HBV markers (HBVm). Conclusion The detection sensitivity of HBV DNA in PCR assay was higher than Dot-H method. The detection rate of HBV DNA was elevated by the various primers in used PCR.
出处
《咸宁医学院学报》
1998年第4期216-219,共4页
Journal of Xianning Medical College
