摘要
目的：探讨神经毒素兴奋性氨基酸（ Ｅ Ａ Ａ）和神经肽在脊髓损伤中的作用。方法：应用高效液相色谱法检测脊髓损伤后伤段组织中谷氨酸（ Ｇｌｕ）、天门冬氨酸（ Ａｓｐ）两种 Ｅ Ａ Ａ 的含量变化；应用放射免疫分析技术测定脊髓损伤伤段组织中神经肽之一强啡肽 Ｄｙｎ Ａ１～１３ 含量的变化；观察了蛛网膜下腔注射 Ｇｌｕ、 Ｄｙｎ Ａ１～１３、兴奋性氨基酸受体拮抗剂３－ （２－ 羧基哌嗪）丙基－ １－ 磷酸（ Ｃ Ｐ Ｐ）、 Ｄｙｎ Ａ１～１３抗血清（ Ａｎ－ Ｄｙｎ Ａ１～１３）对大鼠脊髓损伤的影响。结果： Ｅ Ａ Ａ 和 Ｄｙｎ Ａ１～１３均随损伤程度的加重进行性升高，且呈时间依赖性变化。外源性 Ｇｌｕ 可显著加重脊髓损伤的严重程度， Ｄｙｎ Ａ１～１３ 可产生剂量相关的后肢瘫痪。而 Ｃ Ｐ Ｐ和 Ａｎ－ Ｄｙｎ Ａ１～１３对大鼠脊髓继发性损伤有保护作用。结论：实验结果提示 Ｅ Ａ Ａ、 Ｄｙｎ Ａ１～１３作为神经毒素参与脊髓损伤后的继发性损伤过程。
Objective:To elucidate the effect of neurotoxin,excitatory amino acid and neuropeptide on spinal cord injury post trauma.Methods:Changs in dynorphin DynA 1～13 ,an endogenous opiod,immnoreactivity following traumatic spinal cord injury in the rat were examined.We observed the effects of Glu and dynorphin DynA 1～13 administered intrathecally on function of spinal cord in rats and the effects of 3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid(CPP),an antagonist of EAA receptor,and antisera against dynorphin adminstered intrathecaly on secondary spinal cord injury.Results The concentrations of EAA and dynorphin DynA 1～13 increased progressively along with the severity of injury,and were related to injury time.Glu exacerbated the extent of spinal cord injury.Dynorphin DynA 1～13 were found to have protective effect on spinal cord injury.Conclusion These findings further support the potential pathophysiological role of EAA and dynophin DynA 1～13 as neurotoxin in spinal cord injury.
出处
《实用骨科杂志》
1999年第3期145-148,共4页
Journal of Practical Orthopaedics
