摘要
在纤维蛋白原(简称纤原)常规生产工艺中增加“有机溶剂/表面活性剂(S/D)”灭活病毒步骤·TNBP及Tween-80浓度分别为0.3％及1％,在24℃处理6小时,能有效灭活标志病毒VSV(7.31g_(10))及Sindbis V(6.831g_(10))。残余的TNBP及Tween-80可通过两次8％乙醇沉淀去除。病毒灭活后纤原的可凝固蛋白纯度由原来的70.9±8.5％提高至85.5±6.0％,回收率可保持在64.7±3.8％。PAGE电泳显示来出现新的蛋白区带。
Conventional technology of preparing fibrinogen was improved by developing a procedure of virus inactivation by S/D. Marker viruses, VSV and Sindbis, could be effectively inactivated by 0.3% TNBP and 1% Tween-80 at 24℃ for 6 hours. After inactivation, the titers of two viruses were 7.31g10 and 6. 831g10, respectively. The residual TNBP and Tween-80 could be eliminated by twice precipitation with 8% ethanol. After virus inactivation, the purity of coagulable protein of fibrinogen was improved from 70.9±8. 5% to 85. 5±6. 0%, and the recovery rate was maintained at 64.7±3.8%. Detecting by PAGE, no new protein band was found.
出处
《中国生物制品学杂志》
CAS
CSCD
1993年第3期118-121,共4页
Chinese Journal of Biologicals
关键词
纤维蛋白原
灭活病毒
制剂
Fibrinogen Virus inactivation S/D treatment
