摘要
用抗人鼻咽癌单抗FC2 ,HNL5 (Ab1)分别免疫Balb/C小鼠 ,制备了两株抗独特型抗体 2H4和 5D3(Ab2 )。结果显示 :2H4和 5D3分别与FC2和HNL5特异性结合 ,抑制Ab1与靶细胞的反应 ,诱导产生能与相应Ab1竞争结合靶抗原的抗抗独特型抗体 (Ab3) ;并能诱发特异性迟发型超敏反应 (DTH)和细胞增殖反应。表明 2H4和 5D3具有鼻咽癌相关抗原的内影像 (Ab2 β) ,能模拟鼻咽癌相关抗原诱导体液和细胞免疫反应。
We prepared anti idiotypic monoclonal antibodies (Ab2) against FC2 and HNL5 that recognized as nasopharyngeal cancer (NPC) associated antigens. These Ab2, which designated as 2H4 and 5D3, could inhibit the binding of FC2 or HNL5 to NPC cell lines. The anti sera from the immunized mice that contained anti anti idiotype antibodies (Ab3) could compete with FC2 or HNL5 for binding with NPC cell by a competitive inhibition assay. When 2H4 or 5D3 immunized mice coupled with keyhole limpet hemocyanin(KLH), positive and specific delayed type hypersensitivity (DTH) reaction occurred after the mice were stimulated by NPC cells.The proliferative response of the splenocytes was significantly higher in experimental groups than that in control groups. Therefore, it is suggested that the anti idiotypic antibodies (Ab2) 2H4 and 5D3 are the Ab2 beta bearing the internal image of the epitope of NPC associated antigen, that either 2H4 or 5D3 expressing three dimensional shapes which resemble the structure of natural antigens can induce humoral and cellular immune response. [
出处
《湖南医科大学学报》
CSCD
2000年第4期319-322,共4页
Bulletin of Hunan Medical University
基金
国家自然科学基金!资助项目 ( 3 92 70 716)
关键词
鼻咽肿瘤
癌
抗独特型抗体
抗-抗独特型抗体
迟发型超敏反应
细胞增殖
nasopharyngeal neoplasms
carcinoma
anti idiotypic antibody *
anti anti idiotypic antibody *
delayed type hypersensitivity *
cell proliferation
