摘要
本研究采用辐照甘薯切苗,接种单节段,离体培养并筛选变异体。以三种不同基因型作辐射材料。初步明确了辐射和组培结合过程中一些基本技术条件,如培养基、基因型、光照条件、组培条件下的辐照剂量等,初步获得薯皮色变异系,对线虫性糠腐病抗扩展的变异系。它比常规辐射育种可节省大量田间工作量,并能缩短育种周期,在研究实践中发现,接种单节段(经辐照)最后长成的苗,并非都是一次性地从原腋芽长成,而能从腋芽停止生长1—2周后再由新芽长成小植株。对照则直接从原腋芽长出。
Cuttings from 3 different genotypes of sweet potato were irradiated, cut into singlenode and cultured on specific culture medium in order to select expected mutants.Some foundamental problems about the tissue culture of irradiated sweet potato werepreliminarily solved, including the suitable genotype, culture medium, light and tempera-ture conditions and dosage of irradiation. A mutant line of skin color of tuber rootwas obtained and so was a variant resistant to nematode. Through this approach alot of field labour could be saved and the breeding Cycle shorten, It was also foundthat the tube cultured new plantlet did not grow directly from the axillary bud of theirradiated cuttings in the tube; the bud usually grow for a certain period and thenstopped for 1--2 weeks; a new axillary bud would be differentiated and a newplantlet would grow from the new bud.
出处
《作物学报》
CAS
CSCD
北大核心
1993年第4期309-314,共6页
Acta Agronomica Sinica
基金
FAO/IAEA资助
关键词
甘薯
组织培养
突变体
选择育种
Irradiation
In vitro culture
Single node
Axillary bud
