摘要
本文将高效薄层色谱法和常规薄层色谱法的程序多次展开技术和小孔线形技术用于生脉饮中难分离物质五味子醇甲的分离研究。结果表明:常规薄层小孔线形分离技术具有实用、简便和快速的特点;生脉饮经乙醚萃取后,硅胶GF薄层分离,薄层扫描法测定五味子醇甲的回收率达99.4%。
Sheng Mai Yin consisting of Codonopsis pilosula Nannf., Schisandra chinensis Baill and Ophiopogon japonicus Ker-Gawl is widely used in traditional Chinese medicine. The separation efficiency of programmed multiple development (PMD) and small hole in line (SHL) technique in HPTLC and TLC utilized for the separation of Wuweizi alcohol A (Schisandrin), which is one of the active ingredients of lignans of Schizandra chinensis contained in Sheng Mai Yin, has been studied. The SHL technique in TLC is simple, rapid and practical. The method of quantitative determination has been also established by extraction with ether-separation using PMD or SHL technique in silica GF_(254) TLC-densitometry in this paper. The recovery was 99.4% (n=4).
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1993年第2期78-81,共4页
Journal of China Pharmaceutical University
关键词
生脉饮
五味子醇甲
高效薄层色谱
Sheng Mai Yin
Wuweizi alcohol A
High performance thin layer chromatography
Thin layer chromatography
Programmed multiple development
Small hole in line technique
Densitometry
