摘要
本文采用5′-核苷酸酶-碱性磷酸酶(5′-Nase-AlPase)双重染色法观察了家兔、豚鼠和大鼠胃壁淋巴管的微细分布。在光镜下见毛细淋巴管和淋巴管显示5′-Nase反应强阳性,而毛细血管和血管则显示AlPase反应强阳性。表明3种动物的胃壁各层内均有毛细淋巴管分布,而淋巴管只存在于粘膜下层、肌层和浆膜层。粘膜层的毛细淋巴管主要分布在固有膜的深层,有时见于粘膜肌内,但在上皮层及上皮层下均未发现毛细淋巴管。粘膜下层中的毛细淋巴管较多位于靠近粘膜层一侧,而淋巴管则多靠近肌层一侧。在肌层内,毛细淋巴管和淋巴管不仅存在于不同走行的3层肌间,而且存在于每层内的肌纤维束之间。在浆膜层有较大的淋巴管。本研究同时做了光镜半薄切片和电镜观察,证实了上述的淋巴管分布。3种动物胃壁淋巴管的微细分布未见到种属间差异。
By using 5'-nucleotidase-alkaline phosphatase (5'-Nase-AlPase) double staining method, the fine distribution of the lymph vessels in the stomach wall of the rabbit, guinea pig and rat has been observed. Under light microscope lymph capillaries showed strongly 5'-Nase positive reaction with brown or dark brown staining. However blood capillaries revealed significantly A1Pase activity with blue staining. The lymph capillaries were seen in all layers of the stomach wall in three species, but the lymph vessels were only shown in the submucosa, muscularis and serosa. In the mucosa lymph capillaries could be found in the deep layer of the lamina propria. In the submucosa there were lymph vessels and capillaries. In the muscularis lymph capillaries and vessels not only could be found between oblique and circular muscle or between circular and longitudinal muscle, but also among muscle fiber bundles of each layer. In the serosa there were larger lymph vessels situated near the muscularis. The observation on semithin and ultrathin sections also shown the similarity in the lymph vessels distribution. There was no difference in the distribution of lymph vessels in the stomach wall between rabbit, guinea pig and rat.
出处
《解剖学报》
CAS
CSCD
北大核心
1993年第1期97-100,T017,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助课题
关键词
胃
淋巴管
核苷酸酶
碱性磷酸酶
Stomach
Lymph vessels
5'-Nucleotidase-alkaline double staining method
Rabbit
Rat
Guinea pig
