摘要
用免疫组织化学ABC技术,观察了猫延髓吻侧腹外侧区(RVL)5-羟色胺(5-HT)、苯乙醇胺氮位甲基转移酶(PNMT)、P物质(SP)、亮氨酸-脑啡肽(L-ENK)免疫反应神经元的分布,并用免疫荧光组织化学法结合荧光素逆行追踪法初步研究了RVL内5-HT、SP、L-ENK免疫反应神经元向胸髓的投射。结果表明:4种物质的免疫反应神经元主要分布在旁巨细胞外侧核的尾侧部和外侧网状核的吻侧部。SP阳性细胞还见于疑核腹外侧紧邻的网状结构中。有的5-HT、SP和L-ENK阳性细胞位于紧靠软脑膜的区域。在RVL的大部分区域,4种物质的免疫反应细胞呈重叠分布。在斜方体尾侧1.0～3.5mm平面,距中线约3.3mm,距腹面约0.8mm的区域,5-HT、PNMT和L-ENK阳性神经元均聚集成团,突起相互交织,在吻尾方向上各自形成长短不一的连续细胞柱。这3条细胞柱在斜方体尾侧1.5～2.5mm平面几乎完全重合。RVL部分5-HT、SP和L-ENK神经元发出纤维投射至胸髓。本文还就上述物质在此区的功能意义进行了讨论。
The distribution of serotonin(5-HT), phenylethanolamine-N-methyltransferase (PNMT), substance P(SP) and leu-enkephalin(L-ENK) immunoreactive neurons in the rostral ventrolateral medulla (RVL) of the cat was studied with the immunohistochemical ABC technique, and the projection of 5-HT, SP and L-ENK positive neurons of the RVL into the thoracic cord was preliminarily investigated by a combined fluorescent retrograde transport and immunofluorescence method. The results indicate that 5-HT, PNMT, SP and L-ENK immunoreactive neurons mentioned above were localized primarily in the caudal part of nucleus paragigantocellularis lateralis and the rostral part of nucleus lateralis reticularis. SP positive cell bodies in the reticular formation close ventrolateral to nucleus ambiguus were also found. Some 5-HT, SP and L-ENK positive cells were situated in the area near the pia mater. In the most area of the RVL, 5-HT, PNMT, SP and L-ENK immunoreactive cell bodies had an overlapping distribution. 5-HT or PNMT or L-ENK positive neurons crowded, intertwined each other with their processes in the region at the levels 1.0-3.5mm caudal to trapezoid body, about 3.3mm lateral to the midline and about 0.8mm from the ventral surface of the medulla, and formed a longer or shorter continuous cell column which ran in the rostrocaudal direction. These three columns nearly coincided with each other at the level 1.5-2.5mm caudal to trapezoid body. Part of 5-HT, SP and L-ENK positive neurons in the RVL projected into the thoracic cord. The functional significance of these substances in the RVL was also discussed.
出处
《解剖学报》
CAS
CSCD
北大核心
1993年第3期254-259,T005,共7页
Acta Anatomica Sinica
关键词
延髓
血清素
神经肽
免疫组织化学
Rostral ventrolateral medulla
Serotonin
Phenylethanolamine-Nmethyl-transferase
Neuropeptide
Immunohistochemistry
Cat
