摘要
本文报道了一种单用琼脂糖(Sepharose4B)来纯化蓖麻毒蛋白的快速简便的方法。我们发现在pH5的条件下,蓖麻毒蛋白和与其密切相关的蓖麻凝集素对琼脂糖的结合能力有很大的差别。在有0.2mol/LD-半乳糖存在下可将蓖麻毒蛋白从Sepharose上洗下,同样条件下蓖麻凝集素仍牢固地结合在柱上。从而经一步柱层析便可得到电泳纯的蓖麻毒蛋白。此法不需另行合成亲和胶,适合于蓖麻毒蛋白的大规模纯化。
A simple and time-saving method for the purification of ricin using Se-pharose 4B is reported in this paper. We have discovered that there was great difference between the binding abilities of ricin and its related lectin,ricinus agglutinin to Sepharose 4B at pH5.0. In the presence of 0.2 mol/L D-galactose ricin could be eluted from a Sepharose 4B while the agglutinin was sticked on the column, thereby obtaining pure ricin in one-step chromatography.It is not required to synthesize a special affinity-chromatographic matrix in this method which is particularly useful for the large-scale isolation of ricin.
