摘要
放射性核素^（１３１）Ｉ标记的抗人小扁豆凝集素结合型甲胎蛋白异质体（ＡＦＰ－Ｒ－ＬＣＡ）ＭｃＡｂ，注入荷瘤裸鼠腹腔后，能在裸鼠人肝癌模型肿瘤区积聚，其放射性核素浓度是裸鼠肝脏的５．２倍；而^（１３１）Ｉ标记的正常小鼠ＩｇＧ（ｍＩｇＧ）及游离^（１３１）Ｉ却无肿瘤区积聚，且在荷瘤裸鼠体内呈均匀分布。^（１３１）Ｉ－ＡＦＰ－Ｒ－ＬＣＡＭｃＡｂ组γ照像均显示出裸鼠人肝癌的阳性定位。用^（１３１）Ｉ－ＡＦＰ－Ｒ－ＬＣＡＭｃＡｂ治疗裸鼠人肝癌，肿瘤生长受到明显抑制（８５．０％），优于^（１３１）Ｉ－ｍＩｇＧ及游离^（１３１）Ｉ（４６．０％、２０；１％，Ｐ＜０．０５）．结果表明：ＡＦＰ－Ｒ－ＬＣＡＭｃＡｂ对人肝癌细胞有较强的亲和力和特异性，有希望成为肝癌导向研究的理想载体。
Monoclonal antibody against anti-human AFP variant (AFP-R-LCA McAb) was labeled by(131)I and injected intraperitoneally.The (131)I-AFP-R-LCA McAb was gathered in tumorof nude mice bearing human HCC.The tumor/liver radioactivity ratio was 5.2,but normal mice IgG labeled ty (131)I(131-mIgG)or(131)I was not gathered in tumor and distributed everywhere on an average.The tumors in the group injected with (131)I-AFP-R-LCA McAb were positively visualized by gamma camera.The test showed that the inhibitory rate of tumor growth(85.0%) in the group treated with (131)I-AFP-R-LCA McAb at the 4th week after treatment was significantiy higher than those of the other groups(46.0%,20.1%,respectively,P<0.05).The results show that the AFP-R-LCA McAb has a strong affinity and special binding of HCC eells and it can be recognized as a carrier for radioimmuno-detection and radioimmuno-therapy.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1994年第5期446-451,共6页
Academic Journal of Second Military Medical University
关键词
癌
甲胎蛋白
单克隆抗体
放射免疫测定
肝肿瘤
hepatocellular carcinoma
AFP
McAb
radioimmunodetection
radioimmunotherapy
nude mice
