摘要
作者应用免疫金银染色（ＩＧＳＳ）方法检测了８２例胃癌标本ｐ５３，ｒａｓｐ２１，Ｃ－ｅｒｂＢ－２癌基因蛋白。其阳性结果分别为５６．１％，５１．２％，５８．５％，胃癌旁正常粘膜上皮的阳性率分别为１．２％，１４．６％，９．８％，与肿瘤区相比较有显著差异（Ｐ＜０．０１）。ｐ５３阳性细胞的染色部位在细胞核内，ｐ２１及ｐ１８５阳性细胞的染色部位在胞浆及／或胞膜。ｐ５３、ｐ１８５阳性表达与肿瘤的分化程度有关，即分化越差，其阳性表达越高。８２例胃癌中有３６例显示ｐ５３与ｐ２１协同阳性。而无１例单独ｐ５３表达阳性。
Eighty two cases of human gastric cancer were examined to observe expression and distributive feature of p53, p21 and p185 oncoproteins with immunogold silver staining(IGSS) method. The positive rate of expression was respectively 56. 1%(46/82) for p53; 51.2%(42/82) for p21 and 58.5%(48/82) for p185; and that in the gastric mucomembrane nearby carcinomas was respectively 1.2%(1/82) for p53; 14.6%(12/82) for p21 and 9.8%(8/82) for p185. The different expression of p53, p21 and p185 oncoproteins in carcinoma and non carcinoma tissues were very obvious(P<0.01). The expression of p53 and C-erbB-2 position was related to the differentiation of tumour tissues, and appeared only in the carcinoma tissues. The positive cells of p53 oncoprotein appeared in nuclei of tumour cells, while the positive cells of p185 and p21 oncoproteins appeared in the membrane or plasma of tumour cells.
出处
《第二军医大学学报》
CSCD
北大核心
1994年第1期20-24,共5页
Academic Journal of Second Military Medical University
关键词
免疫金银染色
癌基因
胃肿瘤
oncoproteins
immunogold silver staining
gastric cancer
patients
