摘要
氧化低密度脂蛋白（Ｏ－ＬＤＬ）对巨噬细胞（Ｍｐｈ）有很强的细胞毒作用，能抑制Ｍｐｈ的免疫反应能力，造成脂肪堆积，使Ｍｐｈ泡沫样变性。云芝多糖（ＰＳＫ）能提高机体免疫反应能力。我们研究发现Ｍｐｈ在体外与Ｏ－ＬＤＬ共同培养一段时间后形态有很大改变，如正常梭形伪足消失，细胞变大变园，周边有颗粒沉积，并出现空泡，同时受Ｏ－ＬＤＬ攻击的Ｍｐｈ用ＬＰＳ刺激，ＴＮＦ和ＮＯ产生量都较未受Ｏ－ＬＤＬ攻击的要少（Ｐ＜０．０１），而腹腔注射ＰＳＫ的小鼠Ｍｐｈ在体外与Ｏ－ＬＤＬ共同培养２４ｈ后细胞形态未见有较大改变，并且受ＬＰＳ刺激受ＴＮＰ和ＮＤ产生量都较注射生理盐水对照组要高。因此ＰＳＫ能提高Ｍｐｈ免疫反应能力和抑制其泡沫样变性，这对抑制动脉粥样硬化的进程有益处。
-LDL has high cytotoxity to macrophage. It not only
inhibits the capabihlity of the cells immune responsiveness,but also caus-es lipid accumulation
and foam-like change. Our studies found that after macrophages were preincubated with O-LDL
for 24h, theirshapes change notably. When stimulated by LPS, the production of TNF and NO is
less than that of normal control group, which hasnot been incubated with
O-LDL(p<0.01).However, the shape of PSK-treated macrophages did not change after incubating
with O-LDL for 24h. Moreover,when stimulated by LPS, the macrophages produced more TNF
and NO than the saline-treated immune re-sponse and inhibits their foam-like change.
出处
《第一军医大学学报》
CSCD
1994年第1期12-14,共3页
Journal of First Military Medical University
关键词
低密度脂蛋白
云芝
多糖
巨噬细胞
oxdized-LDL
tumor necrosis factor
nitric oxide
polysacharibe
