摘要
通过三氯乙酸改变细菌视紫红质的微环境而使其完全变性，测定了细菌视紫红质微环境改变前、改变过程中及完全变性后的表面光电压谱、吸收光谱、圆二色谱及荧光光谱的变化，探讨了三氯乙酸对细菌视紫红质电子光谱的影响．
A kind of protein denaturant──CCl3COOH was used to change the microenvironment of bacteriorhodopsin gradually and resulted in bacteriorhodopsin denaturation finally.The surface photovoltage spectrum, adsorbance spectrum, circular dichroism spectrum and fluorescence spectrum were determined during the processes of changing the microenvironment of bacterorhodopsin, and the effect of trichloroacetic acid on the electronic spectra of bacteriorhodopsin was discussed.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1994年第8期1217-1219,共3页
Chemical Journal of Chinese Universities
基金
酶工程国家重点实验室资助
关键词
细菌视紫红质
三氯乙酸
电子光谱
Bacteriorhodopsin,Trichloroacetic acid,Electronic spectra, Surface photovoltage spectra
