摘要
从萌发的水稻种子中分离并纯化了一种能抑制多种病原真菌生长的蛋白（简称为ＲＡＦＰＩ），在马铃薯葡糖琼脂培养基上，２０μｇＲＡＦＰＩ可明显抑制木霉（Ｔｒｉｃｈｏｄｅｒｍａｒｅｅｓｅｉ）菌丝的生长，此蛋白还具有较广的抗真菌菌谱，其分子量为１６．５９ＫＤ，等电点为８．７，组成成分中富含Ｐｒｏ、ＧｌＸ和Ａｓｘ，经测序得到了其Ｎ端２０个氨基酸序列。
n anti-fungal protein named RAFPI is isolated from germinating rice grains. It can strongly inhibitthe growth of Trichoderma reesei on cultured medium, as well as of several other pathogenic fungi. Itsmolecular weight is about 16. 59KD and itS pI= 8. 7. This protein is rich in Pro, Alx and Glx. The 20amino acid residues at the N-terminal are also determined.
出处
《高技术通讯》
EI
CAS
CSCD
1994年第2期22-26,共5页
Chinese High Technology Letters
基金
国家自然科学基金会和863计划资助项目
关键词
抗真菌蛋白
水稻
分离纯化
: Antifungal protein, Rice, Isolation and purification
