摘要
干扰素（IFN）是某些生物体细胞抗病毒感染的一类蛋白质，可用于治疗病毒性疾病和某些癌症。但由人血液获得的干扰素其量极微，目前已能通过基因工程大量生产。编码干扰素的基因已能在大肠杆菌~[1,3]、醇母~[4,5]和哺乳动物细胞表达。~[6]但仍需对工程菌的表达规律作进一步研究，特别要弄清各种因素对被克隆的外源基因表达的影响。
By transforming plasmid pBSM22 and pBV37 which could express human in-terferon a1 into several E. coli strains with different genetic characteristics, a new and better host cell was found. The new cloned strains produced 2-4 fold interferon to the old ones. One of the reasons for the differance of interferon expressed by those cloned strains was that there were different levels of lac repressors among these host cells. The higher the concentration of lac repressor, the lower the interferon activity would be. There were still distinction in expression levels among the cloned strains after IPTG was added, suggesting other mechanisms by which the host cells affected expression of plasmids. It was also found that the reported enhancing effect of SV40 HindIII B fragment on human interferon gene expression in prokaryotic cells was subject to not only the construction of plasmids but also the genetic characteristics of host cells.
出处
《工业微生物》
CAS
CSCD
北大核心
1994年第4期6-10,共5页
Industrial Microbiology
