摘要
从人参培养细胞的细胞壁中分离纯化到不同分子量的单体人参寡糖素。试验结果表明命名为人参寡糖素Ⅶ和人参寡糖素Ⅷ的两种寡糖素对三七悬浮培养细胞的生长具有明显的促进作用，其增长率分别为１９．３４％和１０．５８％，人参寡糖素Ⅶ的适宜浓度为５—１０ｍｇ／ｌ。在高浓度下（大于２５ｍｇ／ｌ）稍抑制培养细胞生长。在细胞培养２２天（指数生长期）后．加入１０ｍｇ／ｌ的人参寡糖素Ⅶ．然后再培养２天。其生长速率即提高，加入人参寡糖素Ⅷ后．缩短了三七细胞悬浮培养生长的延缓期．提前进入对数生长期和指数生长期，并在对数生长期和指数生长期作用最明显，因而最终收获时培养细胞的产率增加。
Different kinds of pure single ginseng-oligosaccharins were isolated andpurified from the Culture Cell walls of Panax ginseng. The study results found thatthe two of these oligosaccharins which were named ginseng-oligosaccharin Ⅶ andginseng-oligosaccharinⅦ could significantly promote the growth rate (which increased by 19.24% and 10.58%respectively) of suspension culture cells from Panaxnotoginseng. The optimum concentration of ginseng-oligosaccharinⅦon culture cellgrowth was 5-10 mg/l. But the culture cell growth was inhibited in a little casewhen its concentration was above 25 mg/l. The culture cell growth rate began toincrease after added ginseng-oligosaccharinⅦ(10mg/l) in the suspension cells whichhad been incubating for 22 days (the growth was in the phase of exponential growth)for 2 days. After added ginseng-oligosaccharinⅦ, the phase of delay growth ofsuspension culture cells in the growth curve was reduced, so the phases of logarithmic and exponential growth were come in advance. The effects of ginseng-olthesaccharinⅦ on cell growth were most significant in the phases of logarithmic andexponential growth, so the yield of culture cells was increased in the harvest time.
出处
《广西植物》
CAS
CSCD
北大核心
1994年第1期70-73,共4页
Guihaia
基金
国家"七五"攻关课题
关键词
人参
寡糖素
三七
细胞悬浮培养
Ginseng-oligosaccharins
Panax notoginseng
cell suspension culture
growth rate: cell yield
