摘要
本文用聚丙烯酰胺凝胶电泳，对Ｏｓｔｒｉｎｉａ属５种近缘种４种酶的同工酶分析后发现亚洲玉米螟、欧洲玉米螟、宁夏苍耳螟和豆螟的同工酶均具多态现象。根据酶谱的差异，绘出了Ｏｓ－ｔｒｉｎｉａ属５种近缘种的亲缘关系图，并利用Ｎｅｉ，Ｍ的遗传距离计算公式求得亚洲玉米螟和欧洲玉米螟之间的遗传距离是１．２１９，亚洲玉米螟与宁夏苍耳螟间的遗传距离为１．０４６，欧洲玉米螟与宁夏苍耳螟间为０．２１６。根据亚种间遗传距离在０．２３左右的意见，作者首次认为宁夏苍耳螟是欧洲玉米螟的一个亚种。
Four kinds of isoenzymes(Est,MDH,LDH,ADH)of the five related species ofOstrinia were studied with polyacylamide gel electrophoresis,and polymorphiam was foundin the zymograms of Asian corn borer O. furnacalis(Guenee),European corn borer O. nubi-lalis(Hubner)and Ningxia siberian cocklebur pyralid O. spp.Their sibship’s drawings weremade according to the zymorgrams. With Nei’s formula,it was calculated that the geneticdistance between Asian corn borer and European corn borer was 1.219,the one betweenAsian corn borer and siberian cocklebur pyralid in Ningxia was 1.046,and the one betweenEuropean corn borer and Ningxia siberian cocklebur pyralid was0.216.In regard to the ge-netic distance,Ningxia siberian cocklebur pyralid should be regarded as a subspecies of Eu-ropean corn borer Ostrinia nubilalis(Hubner)。
出处
《河南农业大学学报》
CAS
CSCD
1994年第1期1-7,共7页
Journal of Henan Agricultural University
关键词
秆野螟属
近缘种
同功酶
分类
Ostrinia
releted species
Corn borer
classification
isoenzyme
electrophoresis
