摘要
利用人胎肝脏为材料,通过匀浆、高速离心、凝胶层析等方法分离、纯化得到线粒体谷丙转氨酶,经聚丙烯酰胺凝胶电泳证实达到电泳纯,并对其理化性质进行了探讨。
The paper has isolated and purified the mitochondric glutamic pyruvic transaminase (GPTm) with the method of homogenate, high-centrifugation, Sephadex G-75, Sephadex G-100 gel colum chromatography from the fetal human liver. The GPTm is the purification of polyacrylamide gel electrophoresis. Some physical and chemical property of GPTm were alsostudied.
关键词
线粒体
转氨酶类
血液
化学
mitochondria
aminotransferases/BL
aminotransferases/CH