摘要
用不同浓度的叠氨化钠处理小麦种子，研究Ｍ＿１代种子萌发和幼苗生长过程中性状和酶活性的变化。结果表明，在０．２到２．０ｍｍｏｌ·Ｌ￣（－１）浓度范围内，叠氮化钠抑制种子萌发和生长，抑制去氢酶和过氧化物酶活性随萌发过程的增高。低浓度叠氮比物（０．００２ｍｍｏｌ·Ｌ￣（－１））处理刺激幼苗生长，提高酶的活性，在一定萌发时期内，去氢酶活性和过氧化物酶活性与幼苗高度显著正相关，２ｍｍｏｌ·Ｌ￣（－１）叠氮化钠处理，Ｍ＿１代结实率为８１．５％。叠氨化物不改变酯酶同工酶酶带数目和迁移率，但对酶带颜色有一定影响。
The changes of characters and activities of ebzymes of M_1 wheat seed were studied during germi-nation and seedling growth fllowing the trdatments of sodium azide of several concentrations.The experimcntal re-sults showed that within the concentration extents from 0.2 to 2.0mmol·L￣(-1),sodium azide inbibited germina-tion and seedling growth,and increases of enzyme activities along with germination course.Low concentration azide treatment(0.002 mmol·L￣(-1))stimulated seedling growth and enbanced enzyme activities.With a certaingermination pericd,activities of dehydrogenase and peroxidase correlated positively with seedling height significant-ly.The azide solution of the highest concentration resulted in 81.5%fertioity in M_1 generation .Sodium azide hadno effect on the quantity of dsterase isoenayme but changed the intensities of some isoenaymes.
出处
《湖北农学院学报》
1994年第3期56-60,共5页
Journal of Hubei Agricultural College
关键词
小麦
叠氮化钠
诱变效应
wheat(T.aesliuum L.)
Sodium azide
Mutagenic effect
